Estrogen and progesterone receptors in the uterus of the vervet monkey

Journal of Receptor Research
H C PotgieterJ J van der Watt

Abstract

Experimental conditions for the optimal measurement of estrogen (ER) and progesterone (PR) receptors in normal vervet monkey (Cercopithecus aethiops pygerythrus) uteri are described. The uteri of this primate were found to contain relatively high concentrations of both ER and PR. Levels of ER ranged from 151 to 822 femtomoles per mg protein (mean for group assayed is 327 +/- 165 femtomoles per mg protein). PR assays were performed on the same cytosols and the levels ranged from 444 to 2267 femtomoles per mg protein (mean of 1285 +/- 511 femtomoles per mg protein). Mean Kd values for the ER- and PR-ligand complexes were found to be 3.15 +/- 1.4 X 10(-10)M and 2.38 +/- 0.2 X 10(-9)M respectively, within the group analysed (n = 21). The ratio of PR to ER varied between 1.1 and 13.1 with a mean of 4.5 +/- 2.4. Ligand specificity studies revealed that [3H]-17 beta-estradiol binding to the ER could only be inhibited by estrogens or estrogen analogues. The PR however exhibited an affinity for a wider range of ligand types. In low ionic strength buffers both ER and PR sedimented as approximately 8S type molecules in the presence or absence of 10mM sodium molybdate. Both receptors dissociated into smaller components, following a short e...Continue Reading

References

Aug 15, 1979·Biochemical Pharmacology·R F AtenA J Eisenfeld
Jan 1, 1975·Annual Review of Physiology·R M Brenner, N B West
Jul 1, 1975·Journal of Steroid Biochemistry·B M SanbornH S Kuo
Jan 1, 1979·Advances in Experimental Medicine and Biology·E E Baulieu
Feb 1, 1977·Journal of Steroid Biochemistry·C ElsnerG Mikhail
Feb 1, 1977·Journal of Steroid Biochemistry·D V IllingworthG Mikhail
Jan 1, 1974·The Journal of Clinical Endocrinology and Metabolism·L H EvansR Hähnel
Jan 1, 1973·Advances in Experimental Medicine and Biology·D Rodbard
Jan 1, 1972·Annual Review of Biochemistry·E V Jensen, E R DeSombre
Jun 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·D Toft, J Gorski
Jul 26, 1968·Biochemical and Biophysical Research Communications·T Erdos
Feb 1, 1984·Journal of Steroid Biochemistry·M Haukkamaa
Jan 1, 1980·Journal of Steroid Biochemistry·J DelettréJ P Raynaud

Related Concepts

Cercopithecus
Cercopithecus tantalus
Cytoplasmic Matrix
Estradiol, (16 alpha,17 beta)-Isomer
Osmolality
Progesterone, (9 beta,10 alpha)-Isomer
Surgestone
Estrogen Nuclear Receptor
Receptors, Progesterone
Thermodynamics

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Computational Methods for Protein Structures

Computational methods employing machine learning algorithms are powerful tools that can be used to predict the effect of mutations on protein structure. This is important in neurodegenerative disorders, where some mutations can cause the formation of toxic protein aggregations. This feed follows the latests insights into the relationships between mutation and protein structure leading to better understanding of disease.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.