PMID: 444462May 29, 1979Paper

Estrogen-binding proteins of calf uterus. Purification to homogeneity of receptor from cytosol by affinity chromatography

V Sica, F Bresciani


The estrogen receptor has been purified to homogeneity from calf uterus cytosol by sequential affinity chromatography by using heparin--Sepharose 4B and 17-hemisuccinyl-17beta-estradiol-ovalbumin--Sepharose 4B. The procedure yields about 1.2 mg of receptor protein from 1 kg of calf uteri, with a recovery of 53%. The receptor protein, as a complex with 17beta-[3H]estradiol, is purified more than 99%. A single band is seen on polyacrylamide gel ectrophoresis under nondenaturing conditions. 17beta-[3H]Estradiol comigrates with the protein band. As computed from the specific activity of radioactive hormone, 64,450 g of purified receptor protein binds 1 mol of 17beta-estradiol. 17beta-[3H]Estradiol bound to the protein is displaced by estrogenic steriods but not by progesterone, testosterone, or cortisone. As judged by chromatography on calibrated Sephadex G-200 columns, the purified receptor is identical with native receptor in crude cytosol: both show a Stokes radius of 6.4 nm. On sucrose gradient in low-salt buffer, the purified receptor sediments at 8 S. On electrophoresis in NaDodSO4 gels, the purified receptor migrates as a single protein band with an apparent molecular weight of 70,000. The sedimentation coefficient measured ...Continue Reading


Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A M MolinariG A Puca
Jun 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·A A WaldmanJ Goldstein
Jul 11, 1973·Nature: New Biology·V SicaP Cuatrecasas
Jan 1, 1974·Journal of Supramolecular Structure·H P Erickson
Jan 1, 1974·Journal of Supramolecular Structure·J B OlmstedG G Borisy
Jun 20, 1972·Biochemistry·G C Chamness, W L McGuire
Oct 1, 1962·Analytical Biochemistry·T BITTER, H M MUIR

❮ Previous
Next ❯


Jan 1, 1981·Breast Cancer Research and Treatment·L P PertschukN D Bloom
Nov 14, 1979·Biochemical and Biophysical Research Communications·R N Taylor, R G Smith
Jan 1, 1980·Journal of Steroid Biochemistry·G A PucaV Sica
Aug 1, 1981·Journal of Steroid Biochemistry·P LustenbergerM Dautrevaux
Dec 1, 1981·Journal of Steroid Biochemistry·T C Madhok, B S Leung
Mar 1, 1982·Journal of Steroid Biochemistry·G L Greene, E V Jensen
Jun 1, 1984·Journal of Steroid Biochemistry·W A McBlain, G Shyamala
Jan 1, 1986·Journal of Steroid Biochemistry·G L Greene, M F Press
Mar 1, 1986·Journal of Steroid Biochemistry·A D LinstedtR M Brenner
Dec 1, 1988·Journal of Steroid Biochemistry·T RatajczakR Hähnel
Jun 1, 1983·Molecular and Cellular Endocrinology·L H SunF R Frankel
Nov 17, 1980·Biochimica Et Biophysica Acta·W Schneider, M Gschwendt
Jan 1, 1986·Meat Science·M MattioliF Chiesa
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·G L GreeneE V Jensen
May 1, 1980·European Journal of Biochemistry·H M Westphal, M Beato
Jun 26, 1985·Journal of Chromatography·S M HyderJ L Wittliff
Feb 22, 1983·Biochimica Et Biophysica Acta·J R Hubbard, M Kalimi
Feb 1, 1984·Analytical Biochemistry·T R Van OosbreeG C Mueller
Jan 1, 1984·Annals of the New York Academy of Sciences·D J TindallD R Rowley
Mar 1, 1983·Clinics in Endocrinology and Metabolism·K S McCartyK S McCarty
Dec 1, 1980·Analytical Biochemistry·P K SmithG T Hermanson
May 31, 1985·Biochemical and Biophysical Research Communications·S CollinsM A Marletta
Jan 1, 1984·Acta Radiologica. Oncology·H S PoulsenN Brünner
Jan 1, 1983·Journal of Receptor Research·T R Van OosbreeG C Mueller

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Landau-Kleffner Syndrome

Landau Kleffner syndrome (LKS), also called infantile acquired aphasia, acquired epileptic aphasia, or aphasia with convulsive disorder, is a rare childhood neurological syndrome characterized by the sudden or gradual development of aphasia (the inability to understand or express language) and an abnormal electroencephalogram. Discover the latest research on LKS here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.