Estrogen exerts a spatial and temporal influence on reactive oxygen species generation that precedes calcium uptake in high-capacity mitochondria: implications for rapid nongenomic signaling of cell growth

Biochemistry
Jai ParkashD Roy

Abstract

Novel findings that emerged from this study underscore the fact that the dynamic nature of mitochondria leads to functional heterogeneity of [Ca(2+)](mito) with respect to estrogen actions in MCF7 cells. We show that estrogen exposure to cells increased [Ca(2+)](mito) in a high-calcium capacity mitochondrial population but not in low-calcium capacity mitochondria. Physiological concentrations of 17beta-estradiol (E2) modulated Ca(2+)(mito) uptake within 90 s. Interestingly, this calcium response lagged behind the induction of mitochondrial reactive oxygen species (mtROS). The rapid induction of Ca(2+)(mito) in response to E2 and its inhibition by mitochondrial blockers suggest that mitochondria are early nongenomic targets of E2. This suggests that a subpopulation of mitochondria is recruited to respond to new metabolic requirements required by estrogen triggers or, as in this case, E2-induced Ca(2+)(mito) and/or mtROS promotes oxidative signaling without involving nuclear estrogen receptor signaling. Although the early E2-induced Ca(2+) did not alter the expression of genes involved in calcium signaling pathways, an intracellular calcium chelator BAPTA-AM and the Ca(2+)(mito) uniporter blocker ruthenium red prevented E2-induce...Continue Reading

References

Jul 4, 1990·Journal of the National Cancer Institute·P SkehanM R Boyd
May 18, 1999·The American Journal of Physiology·G R Monteith, M P Blaustein
Sep 16, 2000·The Journal of Biological Chemistry·G PaniT Galeotti
Dec 7, 2000·Journal of the National Cancer Institute·R SchiffC K Osborne
Apr 3, 2002·The EMBO Journal·Tony J CollinsMartin D Bootman
Jun 18, 2002·FEBS Letters·Ramune MorkunieneVilmante Borutaite
Dec 24, 2003·British Journal of Pharmacology·M MonteroJ Alvarez
Aug 25, 2004·The Biochemical Journal·Mayte MonteroJavier Alvarez
May 25, 2005·British Journal of Pharmacology·Carmen D LobatónJavier Alvarez

❮ Previous
Next ❯

Citations

Jan 7, 2009·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Timothy J JorgensenKala Visvanathan
Mar 15, 2012·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Jorge Sastre-SerraPilar Roca
Mar 8, 2013·Biochimica Et Biophysica Acta·Rosalind Brigham Penney, Deodutta Roy
Oct 2, 2015·International Journal of Molecular Sciences·Ju-Fang LiuSheng-Mou Hou
Apr 20, 2013·Mitochondrion·Alessandra PagliaraniVittoria Ventrella
Apr 8, 2010·Free Radical Research·Geou-Yarh Liou, Peter Storz
Jun 9, 2006·American Journal of Physiology. Cell Physiology·Cristina Camello-AlmarazPedro J Camello
Dec 14, 2016·Journal of Cellular and Molecular Medicine·Melissa Christine KanderZhenguo Liu
May 5, 2017·International Journal of Endocrinology·Hamza Assaggaf, Quentin Felty
Jul 22, 2021·The FEBS Journal·Ling WuLiang Zhao

❮ Previous
Next ❯

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.