Estrogen, heat shock proteins, and NFkappaB in human vascular endothelium

Arteriosclerosis, Thrombosis, and Vascular Biology
Karyn L HamiltonA A Knowlton

Abstract

We hypothesized that estrogen would increase HSP72 in human coronary artery endothelial cells (HCAEC), and that these would be more sensitive to estrogen than our previous observations in myocytes. HCAEC were treated with 17beta-estradiol or tamoxifen, ranging from physiological to pharmacological(1 nM to 10 micromol/L) for either 24 hours (early) or 7 days (chronic). HSP expression was assessed by Western blots. Both early and chronic 17beta-estradiol and tamoxifen increased HSP72. Electromobility shift assays (EMSA) showed activation of HSF-1 with early, but not chronic, 17beta-estradiol. 17beta-Estradiol activated NFkappaB within 10 minutes, and the ER-alpha selective inhibitor, ICI 182 780, abolished this effect. Transcription factor decoys containing the heat shock element blocked HSP72 induction. Estrogen pretreatment decreased lactate dehydrogenase release with hypoxia. This protective effect persisted despite blockade of HSF-1 by decoys. However, an NF-kappaB decoy prevented the increase in HSP72 and abolished the estrogen-associated protection during hypoxia. 17beta-Estradiol upregulates HSP72 early and chronically via different mechanisms in HCAEC, and provides cytoprotection during hypoxia, independent of HSP72 induc...Continue Reading

References

Jun 20, 1995·Proceedings of the National Academy of Sciences of the United States of America·R MorishitaV J Dzau
Aug 5, 1998·Journal of Molecular and Cellular Cardiology·K SuzukiH Matsuda
Jun 11, 1999·The New England Journal of Medicine·M E Mendelsohn, R H Karas
Oct 12, 2000·Proceedings of the National Academy of Sciences of the United States of America·A NadalB Soria
Dec 21, 2000·American Journal of Physiology. Heart and Circulatory Physiology·A A Knowlton, L Sun
Feb 13, 2001·The Journal of Steroid Biochemistry and Molecular Biology·M E Mendelsohn
Feb 13, 2001·The Journal of Steroid Biochemistry and Molecular Biology·J C Stevenson
Jul 13, 2001·Journal of Molecular and Cellular Cardiology·R N CornelussenA A Knowlton
Aug 11, 2001·Cytokine·R W McMurrayJ K Jenkins
Jul 2, 2002·JAMA : the Journal of the American Medical Association·Deborah GradyUNKNOWN HERS Research Group
Aug 15, 2002·Cardiovascular Research·Kwang Kon Koh
Aug 21, 2002·JAMA : the Journal of the American Medical Association·Heidi D NelsonJanet D Allan
Nov 20, 2002·Circulation·S Gupta, A A Knowlton
Dec 17, 2002·Arteriosclerosis, Thrombosis, and Vascular Biology·Karen J Ho, James K Liao
Dec 17, 2002·The Journal of Biological Chemistry·Paul WebbPeter J Kushner
Apr 26, 2003·American Journal of Physiology. Heart and Circulatory Physiology·M R VossA A Knowlton
Oct 14, 2003·Cardiovascular Toxicology·W Keith JonesMichael McGuinness
Dec 9, 2003·Laboratory Investigation; a Journal of Technical Methods and Pathology·Jian-xiong Chen, Barbara Meyrick

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Citations

Jan 28, 2014·Molecular and Cellular Endocrinology·A A Knowlton, D H Korzick
Dec 31, 2010·Shock·Samuel KobbaAnne A Knowlton
Feb 19, 2005·Pediatric Research·Karin B NelsonEdward Lammer
Feb 10, 2011·Endocrinology·James P SticeAnne A Knowlton
Mar 7, 2014·PloS One·Madeleine Chalfant, Karen K Bernd
Apr 24, 2008·Molecular Medicine·James P Stice, Anne A Knowlton
Apr 18, 2009·Future Cardiology·James P SticeAnne A Knowlton
Feb 27, 2015·Cell Stress & Chaperones·J P HeisermanA A Knowlton
Aug 7, 2008·Journal of Cardiovascular Pharmacology and Therapeutics·Charles L StebbinsAnne A Knowlton
Dec 1, 2018·American Journal of Physiology. Heart and Circulatory Physiology·HyunTae V HwangAnne A Knowlton
Oct 13, 2007·Catheterization and Cardiovascular Interventions : Official Journal of the Society for Cardiac Angiography & Interventions·Alexandre AbizaidEberhard Grube
May 13, 2006·Journal of Applied Physiology·M NickersonM Fleshner
Jun 6, 2015·Molecular and Cellular Biochemistry·Antônio Azambuja MiragemPaulo Ivo Homem de Bittencourt
Nov 8, 2007·Physiological Genomics·Karyn L HamiltonAnne A Knowlton
Dec 1, 2009·Journal of Molecular and Cellular Cardiology·Y WangA A Knowlton

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