Estrogen-induced c-fos protooncogene expression in MCF-7 human breast cancer cells: role of estrogen receptor Sp1 complex formation

Endocrinology
R DuanStephen Safe

Abstract

17Beta-estradiol (E2) induces c-fos protooncogene expression in MCF-7 human breast cancer cells, and previous studies in HeLa cells identified an imperfect palindromic estrogen-responsive element (-1212 to -1200) that was required for trans-activation. In contrast, the estrogen-responsive element was not required for E2 responsiveness in MCF-7 cells, and using a series of constructs containing wild-type (pF1) and mutant 5'-flanking sequences (-1220 to -1155) from the c-fos protooncogene promoter in transient transfection assays, it was shown that a GC-rich motif (5'-GGGGCGTGG) containing an imperfect Sp1-binding site was required for hormone-induced activity. This sequence also bound Sp1 protein in gel mobility shift assays, and coincubation with the estrogen receptor (ER) enhanced Sp1-DNA binding. E2 and 4'-hydroxytamoxifen, but not ICI 164,384, induced reporter gene activity in cells transiently transfected with pF1. E2 induced reporter gene activity in MDA-MB-231 breast cancer cells transiently cotransfected with pF1 and wild-type ER or variant ER in which the DNA-binding domain was deleted (HE11); plasmids expressing N-terminal or C-terminal domains of the ER containing activator function-1 or -2, respectively, were inactiv...Continue Reading

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Citations

Jun 13, 2009·Breast Cancer Research : BCR·Joan S Lewis-Wambi, V Craig Jordan
Jun 5, 2007·PLoS Genetics·Chin-Yo LinEdison T Liu
Sep 6, 2008·Journal of Molecular Endocrinology·Stephen Safe, Kyounghyun Kim
Mar 15, 2006·BMC Molecular Biology·Jiangqin Zhao, Steven J Ennion
Jun 7, 2014·PloS One·Ming-juan DuYoujia Cao
Dec 5, 2015·Frontiers in Immunology·Aleksander Keselman, Nicola Heller
Jan 26, 2011·BMC Cancer·Anita M QuintanaScott A Ness
Oct 16, 2010·PloS One·Aaron R ThornerCharles M Perou
Nov 10, 2012·Gene·Pham Thi Thu HuongBo Yeon Kim
Sep 23, 2008·Biochimica Et Biophysica Acta·Marinella ZilliUNKNOWN Consorzio Interuniversitario Nazionale per la Bio-Oncologia (CINBO)
Apr 23, 2003·Gene·Karen M M KelleyManohar Ratnam
Feb 13, 1999·International Journal of Cancer. Journal International Du Cancer·J M GeeR I Nicholson
Feb 3, 2004·Journal of Cellular Biochemistry·Motoko ShibanumaKiyoshi Nose
Apr 28, 2004·Breast Cancer Research and Treatment·Linda A deGraffenriedSuzanne A W Fuqua
Mar 12, 2005·American Journal of Physiology. Cell Physiology·Naoko Kanda, Shinichi Watanabe
Mar 7, 2006·Journal of Cellular Biochemistry·Hsuen-Tsen ChengWen-Chun Hung
Feb 23, 2008·Molecular Endocrinology·Geneviève Deblois, Vincent Giguère

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