Estrogen-induced proliferation in cultured hepatocytes involves cyclin D1, p21(Cip1) and p27(Kip1).

Digestive Diseases and Sciences
M BaroneA Iolascon

Abstract

The purpose of this study was to establish if estrogen-induced hepatocyte proliferation in vitro involves the cell cycle regulators cyclin D1, p21(Cip1), and p27(Kip1). Male rat hepatocytes were cultured in presence of 17-beta-estradiol (E2) +/- ICI-182780, a pure estrogen antagonist, and [3H]-thymidine, as required. DNA synthesis as well as p21(Cip1), p27(Kip1), and cyclin D1mRNA and protein levels were evaluated at different times (12, 24, 36, and 48 hours) of incubation. E2-increased DNA synthesis was correlated with cyclin D1 and p21(Cip1) (mRNA and protein) variations that were reversed by the addition of ICI-182780. p27(Kip1) protein levels progressively increased regardless of the presence of E2 or ICI-182780. Our data confirm that estrogens' stimulatory effect is related to their ability to increase cyclin D1 levels. The increase of p21(Cip1) is probably related to the reentry of hepatocytes in the quiescent state. p27(Kip1) protein is not able to arrest hepatocyte proliferation.

References

Jan 1, 1976·Methods in Cell Biology·P O Seglen
Nov 22, 1991·Science·B E HendersonM C Pike
Aug 31, 1990·Biochemical and Biophysical Research Communications·E PersicoA Weisz
Aug 1, 1989·Japanese Journal of Cancer Research : Gann·Y HimenoH Imura
Apr 1, 1989·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A FrancavillaV Mazzaferro
Jul 1, 1986·Gastroenterology·A FrancavillaT E Starzl
May 1, 1996·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M BaroneC Panella
Jan 1, 1996·Annual Review of Pharmacology and Toxicology·J D Yager, J G Liehr
Aug 1, 1996·Molecular and Cellular Biology·D AgrawalW J Pledger
Mar 1, 1997·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J H AlbrechtM Y Hu
Sep 10, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·G S Crary, J H Albrecht
Apr 9, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M PibiriA Columbano
Aug 6, 2002·Molecular and Cellular Endocrinology·Erica VillaFederico Manenti
Aug 31, 2002·The Journal of Biological Chemistry·Young Hye KwonAngela L Tyner
Oct 24, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Giovanna M Ledda-ColumbanoAmedeo Columbano
Dec 28, 2002·Molecular Carcinogenesis·Chuanwei YangStewart Sell
Mar 21, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Gennady P IlyinChristiane Guguen-Guillouzo

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Mar 27, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Lisa K MullanyJeffrey H Albrecht

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