Estrogen induces androgen-repressed SOX4 expression to promote progression of prostate cancer cells

The Prostate
Muyi YangBo Han

Abstract

The sex determing region Y-box 4 (SOX4) gene is a critical developmental transcriptional factor that is overexpressed in prostate cancer (PCa). While we and others have investigated the role of SOX4 overexpression in PCa, the molecular mechanism underlying its aberrant expression remains unclear. Immunohistochemistry were utilized to detect SOX4 expression and the correlation between estrogen receptor β (ERβ), androgen receptor (AR) and SOX4 in a cohort of 94 clinical specimens. Real-time quantitative PCR and Western blotting were used to study the transcript and protein expression levels. Immunofluorescence staining and co-immunoprecipitation were performed to assess the interaction and subcellular location of ERβ and AR. Chromatin immunoprecipitation (ChIP) assays and Luciferase reporter assays were performed to explore the binding and transcriptional activities of ERβ and AR to the SOX4 promoter. Cellular function was evaluated by MTS, invasion and wound healing assays. SOX4 expression is up-regulated in Castration-Resistant Prostate Cancer (CRPC) tumors compared to hormone-dependent PCa (HDPC) cases. Increased expression was also observed in PCa cells after long-term androgen-deprivation treatment (ADT). In vitro data indic...Continue Reading

References

Jul 20, 1999·Biology of Reproduction·S M Hunt, C L Clarke
Mar 20, 2002·Nature Reviews. Cancer·B J Feldman, D Feldman
Jun 18, 2005·Genes & Development·William E LowryElaine Fuchs
Nov 10, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Howard I Scher, Charles L Sawyers
Jul 6, 2007·Nature Reviews. Cancer·Stuart J Ellem, Gail P Risbridger
Jul 7, 2007·Physiological Reviews·Nina HeldringJan-Ake Gustafsson
Dec 7, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·William A RickeGail P Risbridger
Jan 11, 2008·Nature·Sohail F TavazoieJoan Massagué
Apr 3, 2008·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Paras B SinghFrancis L Martin
Jan 21, 2009·Human Molecular Genetics·Pedro P MedinaMontse Sanchez-Cespedes
May 2, 2009·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Bo HanRajal B Shah
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Stephen J McPhersonGail P Risbridger
Jan 13, 2012·CA: a Cancer Journal for Clinicians·Rebecca SiegelAhmedin Jemal
Dec 19, 2012·Nature Reviews. Urology·Yang Zong, Andrew S Goldstein
Mar 1, 2013·Endocrine-related Cancer·Natalie SampsonIris E Eder
Jul 18, 2014·Molecular Medicine·Paraskevi ChristoforouMichael Koutsilieris
Sep 3, 2014·Biochemical and Biophysical Research Communications·Weishan WangSanyuan Hu

❮ Previous
Next ❯

Citations

May 18, 2016·SpringerPlus·Karolina Kowalska, Agnieszka Wanda Piastowska-Ciesielska
Sep 13, 2017·Nature Reviews. Urology·Virginia Murillo-Garzón, Robert Kypta
Sep 25, 2019·Cancers·Erika Di ZazzoGabriella Castoria
May 10, 2019·Journal of Mammary Gland Biology and Neoplasia·Gaurav A MehtaMichael L Gatza

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.