PMID: 9419755Jan 1, 1994Paper

Estrogen mustard induces cell cycle arrest of human epithelial ovarian cancer cell lines

Journal of the Society for Gynecologic Investigation
J D NathanF Naftolin

Abstract

Pharmacologic disruption of microtubule function may provide effective therapy for advanced epithelial ovarian cancer, as has been observed in clinical trials using taxol. However, the limited availability of taxol and taxol's side effects emphasize a need to develop alternative antimicrotubule agents. Estramustine (EM) inhibits binding of microtubule-associated proteins (MAPs) to microtubules, promotes microtubule disassembly, disrupts spindle formation, and induces metaphase arrest in human prostate carcinoma and glioma cells in culture. We studied the effect of EM on DNA synthesis and on the cell cycle in four human ovarian carcinoma cell lines and examined the cell lines for evidence of MAP-like immunoreactivity. The effect of EM on DNA synthesis and on the cell cycle was determined using [3H]thymidine incorporation assays and flow cytometry, respectively. Microtubule-associated protein-like immunoreactivity was determined using monoclonal antibodies directed against MAP 1A, MAP 1B, and MAP 2(2A + 2B) for Western analysis after sodium dodecyl sulfate-polyacrylamide gel electrophoresis. We demonstrated a dose-dependent inhibitory response to EM in BIXLER, DK2NMA, and SKOV3. BIX3 showed a dose-dependent inhibitory response to...Continue Reading

References

Jan 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·R D SlobodaP Greengard
Jan 1, 1977·Scandinavian Journal of Urology and Nephrology·G JönssonT Nilsson
Sep 1, 1991·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·E K RowinskyR C Donehower
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Jan 1, 1986·Annals of the New York Academy of Sciences·A Matus, B Riederer

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