Estrogen receptor accessory proteins augment receptor-DNA interaction and DNA bending

The Journal of Steroid Biochemistry and Molecular Biology
C C LandelG L Greene

Abstract

Increasing evidence suggests that accessory proteins play an important role in the ability of the estrogen receptor (ER) and other nuclear hormone receptors to modulate transcription when bound to cis-acting hormone response elements in target genes. We have previously shown that four proteins, hsp70, protein disulfide isomerase (PDI) and two unknown proteins (p48 and p45), copurify with ER that has been isolated by site-specific DNA chromatography (BERE) and influence the interaction of ER with DNA in vitro. To better define the nature of these effects, we used filter binding and electrophoretic mobility shift assays to study the ability of these proteins to alter the kinetics of ER-DNA interaction and to influence the ability of ER to bend DNA when bound to an estrogen response element (ERE). The results of both assays indicate that ERE-purified ER, with its four associated proteins (hsp70, PDI, p48, p45), has a greater ability to bind to the vitellogenin A2 ERE than ER purified by estradiol-Sepharose chromatography in the absence (ESeph) or presence (EATP) of ATP, in which p48, p45 (ESeph) and hsp70 (EATP) are removed. Surprisingly, the rates of association and dissociation of ER and ERE were essentially the same for all thr...Continue Reading

References

Jan 1, 1978·Journal of Steroid Biochemistry·M M Bouton, J P Raynaud
May 1, 1992·Molecular and Cellular Biology·A M Nardulli, D J Shapiro
Jun 30, 1992·Biochemical and Biophysical Research Communications·M SabbahE E Baulieu
Nov 1, 1991·Molecular Endocrinology·T SoneJ W Pike
Nov 22, 1991·Science·T K Kerppola, T Curran
Mar 1, 1990·Journal of Reproduction and Fertility·M G Parker
Sep 1, 1989·Molecular and Cellular Biology·S L KostD O Toft
Jan 1, 1989·Molecular and Cellular Biology·L Klein-HitpassB W O'Malley
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·V CavaillèsM G Parker
Jan 1, 1994·The Journal of Steroid Biochemistry and Molecular Biology·P PrendergastD P Edwards
Oct 1, 1993·Endocrine Reviews·M Pfahl
May 7, 1993·Science·J Pérez-Martín, M Espinosa
Oct 7, 1993·Nature·Y KimP B Sigler
Oct 1, 1995·Environmental Health Perspectives·C C LandelG L Greene
Jan 1, 1997·The Journal of Steroid Biochemistry and Molecular Biology·L N PetzD J Shapiro

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Citations

Dec 10, 2002·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·P LarkinN D Denslow
Oct 9, 2008·The Journal of Steroid Biochemistry and Molecular Biology·Xinmiao FuBao Ting Zhu
Jan 5, 2002·The Journal of Biological Chemistry·Jennifer R SchultzAnn M Nardulli
Apr 28, 2001·Molecular and Cellular Endocrinology·M Szatkowski OzersJ Gorski
Nov 5, 2002·Bioorganic & Medicinal Chemistry·Matthew R LashleyMichael H Nantz

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