Estrogen receptor alpha mediates epithelial to mesenchymal transition, expression of specific matrix effectors and functional properties of breast cancer cells.

Matrix Biology : Journal of the International Society for Matrix Biology
Panagiotis BourisNikos K Karamanos

Abstract

The 17β-estradiol (E2)/estrogen receptor alpha (ERα) signaling pathway is one of the most important pathways in hormone-dependent breast cancer. E2 plays pivotal roles in cancer cell growth, survival, and architecture as well as in gene expression regulatory mechanisms. In this study, we established stably transfected MCF-7 cells by knocking down the ERα gene (designated as MCF-7/SP10+ cells), using specific shRNA lentiviral particles, and compared them with the control cells (MCF-7/c). Interestingly, ERα silencing in MCF-7 cells strongly induced cellular phenotypic changes accompanied by significant changes in gene and protein expression of several markers typical of epithelial to mesenchymal transition (EMT). Notably, these cells exhibited enhanced cell proliferation, migration and invasion. Moreover, ERα suppression strongly affected the gene and protein expression of EGFR and HER2 receptor tyrosine kinases, and various extracellular matrix (ECM) effectors, including matrix metalloproteinases and their endogenous inhibitors (MMPs/TIMPs) and components of the plasminogen activation system. The action caused by E2 in MCF-7/c cells in the expression of HER2, MT1-MMP, MMP1, MMP9, uPA, tPA, and PAI-1 was abolished in MCF-7/SP10+ ...Continue Reading

References

Apr 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·A J WongB Vogelstein
May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·A J EkstrandV P Collins
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A J WongB Vogelstein
Jul 3, 1997·International Journal of Cancer. Journal International Du Cancer·P A AndreasenM J Duffy
Jul 1, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·L RiethdorfT Löning
Oct 28, 1998·Journal of the National Cancer Institute·R M O'ReganV C Jordan
Mar 5, 1999·Breast Cancer Research and Treatment·R W StephensM Schmitt
Jun 8, 1999·Journal of the National Cancer Institute·J MäenpääM Kaukonen
Nov 1, 2000·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·R B ClarkeE Anderson
Oct 13, 2001·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·R I NicholsonM E Harper
Feb 6, 2003·International Journal of Cancer. Journal International Du Cancer·Theoni N MitropoulouNikas K Karamanos
Jul 16, 2003·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·John Mendelsohn, Jose Baselga
Aug 7, 2003·Biochemical and Biophysical Research Communications·Montserrat CaballeroPedro Gonzalez
Nov 6, 2003·International Journal of Cancer. Journal International Du Cancer·Renato BasergaKrysztof Reiss
May 20, 2004·Experimental Cell Research·Boris VuillermozYanusz Wegrowski
Aug 4, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Vasilia-Ismini AlexakiElias Castanas
Aug 26, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Torsten O NielsenCharles M Perou
May 9, 2006·Cancer Metastasis Reviews·Elena I Deryugina, James P Quigley
Mar 14, 2008·The New England Journal of Medicine·Fortunato Ciardiello, Giampaolo Tortora
Jun 20, 2008·The International Journal of Biochemistry & Cell Biology·Joe W Ramos
Jul 1, 2008·The Biochemical Journal·Yin YeSanford H Barsky
May 28, 2009·Cancer Research·Jing LuDihua Yu
Sep 10, 2009·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Roopali RoyMarsha A Moses
Nov 10, 2009·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Jonathan D MarottiRulla M Tamimi
May 14, 2010·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·D Craig Allred
May 12, 2010·Cold Spring Harbor Perspectives in Biology·Geert Berx, Frans van Roy
Jun 15, 2010·Cell Cycle·Sejeong Shin, John Blenis
Nov 20, 2010·The FEBS Journal·Chrisostomi GialeliNikos K Karamanos

❮ Previous
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Citations

Nov 13, 2015·Advanced Drug Delivery Reviews·Achilleas D TheocharisNikos K Karamanos
Feb 10, 2016·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·C N Kent, I K Guttilla Reed
Mar 13, 2016·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Zoi PiperigkouAristidis M Tsatsakis
May 18, 2016·Matrix Biology : Journal of the International Society for Matrix Biology·Zoi PiperigkouNikos K Karamanos
Jun 30, 2016·Matrix Biology : Journal of the International Society for Matrix Biology·Kallirroi VoudouriGeorge N Tzanakakis
Jul 2, 2016·Cell and Tissue Research·Zoi PiperigkouMartin Götte
Jul 21, 2016·Archives of Toxicology·Monica NeaguAristidis Tsatsakis
Oct 25, 2016·Matrix Biology : Journal of the International Society for Matrix Biology·Evgenia KarousouSpyros S Skandalis
Oct 26, 2016·Glycoconjugate Journal·Nikos A AfratisAchilleas D Theocharis
Jun 25, 2017·Biochemical and Biophysical Research Communications·Aura M Jiménez-GarduñoIsaura Meza
Jul 20, 2016·Journal of Clinical Medicine·Joema Felipe LimaJohn M S Bartlett
Sep 22, 2018·Journal of Cellular Physiology·Bagher FarhoodAmirhossein Sahebkar
Aug 21, 2019·Molecular Omics·Virgínia Campos SilvestriniVítor Marcel Faça
Aug 20, 2019·Journal of Experimental & Clinical Cancer Research : CR·Xuefei FengPeijun Liu
May 11, 2016·Bioscience Reports·Yulia Lipovka, John P Konhilas
Jan 7, 2017·Breast Cancer Research and Treatment·Eivind Valen EgelandGunhild M Mælandsmo
Jul 28, 2018·Endocrine·Kristin Astrid Berland ØysteseJens Bollerslev
Mar 27, 2020·OncoTargets and Therapy·Yanfang LiuJing Yao
Aug 23, 2020·Cells·Ana M Hernández-VegaIgnacio Camacho-Arroyo
Aug 2, 2017·Developmental Dynamics : an Official Publication of the American Association of Anatomists·George TzanakakisDragana Nikitovic
Jun 28, 2018·Clinical & Experimental Metastasis·Andrew D RedfernErik W Thompson
Oct 4, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Małgorzata Zakłos-SzydaGrażyna Budryn
Nov 14, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Florian ReinhardtHans Neubauer

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