Estrogen receptor α dependent regulation of estrogen related receptor β and its role in cell cycle in breast cancer.

BMC Cancer
B Madhu KrishnaSandip K Mishra

Abstract

Breast cancer (BC) is highly heterogeneous with ~ 60-70% of estrogen receptor positive BC patient's response to anti-hormone therapy. Estrogen receptors (ERs) play an important role in breast cancer progression and treatment. Estrogen related receptors (ERRs) are a group of nuclear receptors which belong to orphan nuclear receptors, which have sequence homology with ERs and share target genes. Here, we investigated the possible role and clinicopathological importance of ERRβ in breast cancer. Estrogen related receptor β (ERRβ) expression was examined using tissue microarray slides (TMA) of Breast Carcinoma patients with adjacent normal by immunohistochemistry and in breast cancer cell lines. In order to investigate whether ERRβ is a direct target of ERα, we investigated the expression of ERRβ in short hairpin ribonucleic acid knockdown of ERα breast cancer cells by western blot, qRT-PCR and RT-PCR. We further confirmed the binding of ERα by electrophoretic mobility shift assay (EMSA), chromatin immunoprecipitation (ChIP), Re-ChIP and luciferase assays. Fluorescence-activated cell sorting analysis (FACS) was performed to elucidate the role of ERRβ in cell cycle regulation. A Kaplan-Meier Survival analysis of GEO dataset was perf...Continue Reading

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Citations

Feb 29, 2020·International Medical Case Reports Journal·Bahare FazeliHossein Taheri
Oct 10, 2020·Breast Cancer Research and Treatment·Minami HatonoShinichi Toyooka
Dec 21, 2019·Anti-cancer Agents in Medicinal Chemistry·Agata BiniendaElzbieta Pluciennik
Jun 6, 2021·Journal of Cancer Research and Clinical Oncology·Susanne Schüler-ToprakOliver Treeck
Aug 5, 2021·Phytotherapy Research : PTR·Youdan LiangXiaofang Li

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Datasets Mentioned

BETA
GSE9893

Methods Mentioned

BETA
PCR
reverse transcription PCR
MDA
Electrophoretic mobility shift assay
immunoprecipitation assay
ChIP
immunoprecipitation
transfection
fluorescence-activated cell sorting

Software Mentioned

LAS EZ
GraphPad Prism
Image J

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