Estrogen receptor isoform-specific induction of progesterone receptors in human osteoblasts

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
D J RickardT C Spelsberg

Abstract

Estrogen induction of progesterone receptor (PR) expression may be important to bone physiology because progesterone has been implicated in the control of bone formation and resorption. Although PR gene expression can be induced in osteoblasts by estrogen signaling through the estrogen receptor (ER) a isoform, it is unknown whether the ER-beta isoform is involved in this regulation. The effect of estrogen on PR expression was examined in human fetal osteoblast (hFOB) cell lines stably transfected with either ER-alpha or ER-beta. Estrogen treatment of hFOB/ER-a cells induced PR messenger RNA (mRNA) steady-state levels after 24 h and protein levels after 48 h, as established by competitive reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blotting. Interestingly, no induction of PR expression was observed in the hFOB/ER-beta cells during this period. However, PR mRNA was induced progressively after 48 h of treatment with estrogen with maximum levels achieved at 12 days posttreatment. ER protein also was increased after 12 days of treatment. Both A and B isoforms of PR (PRA and PRB) were induced by estrogen in the hFOB/ER-a cells as well as much later in hFOB/ER-beta cells. The pure antiestrogen ICI 182,780 prev...Continue Reading

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Citations

Mar 13, 2013·Journal of Bone and Mineral Metabolism·Bruno MozzanegaGiovanni Battista Nardelli
Mar 12, 2011·Breast Cancer Research : BCR·Xianglin WuJohn R Hawse
Jul 15, 2006·Pharmaceutical Research·Wenqing GaoJames T Dalton
Jul 22, 2015·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Roberta B WolffJosé Maria Soares
Mar 28, 2012·Climacteric : the Journal of the International Menopause Society·V Seifert-KlaussT Wimmer
Jun 2, 2017·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Zhendong A ZhongWei Yao
Jun 3, 2004·Journal of Cellular Physiology·Anne De WildeAlain Pointillart
Dec 14, 2005·American Journal of Clinical Dermatology·Kimberly K Leslie, Eve Espey
Mar 12, 2005·Arquivos brasileiros de endocrinologia e metabologia·Rogéria SerakidesErnane F do Nascimento

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