PMID: 3747726Sep 29, 1986Paper

Estrogen receptors and growth response in cultured human periodontal ligament cells

Life Sciences
W M Lewko, A Anderson

Abstract

The periodontal ligament (PDL) is a connective tissue involved in the remodeling process associated with tooth development and positioning. PDL cells grown in culture were analyzed for the capacity to specifically bind steroid hormones and for growth response to estradiol-17 beta. Using [3H]estradiol-17 beta as the ligand, PDL cells in first passage cultures exhibited a specific estrogen binding capacity of 881 fmol/mg cell protein. With [3H]dexamethasone as a ligand, the binding capacity of the glucocorticoid receptor was 143 fmol/mg protein. With [3H]R5020 as a ligand, the progestin receptor exhibited a binding capacity of 5 pmol/mg protein. Scatchard analysis of estradiol binding at 37 degrees revealed a dissociation constant of 2.7 X 10(-9) M, representative of the estrogen receptor. The addition of estradiol-17 beta at concentrations of 10(-9) and 10(-8) M to culture media induced a dose-dependent decrease in growth (DNA content) to 62% and 38% control values, respectively. The addition of the antiestrogens tamoxifen and 4-hydroxytamoxifen at concentrations of 10(-7) and 10(-6) M similarly depressed cell growth. These results show that PDL cells contain high affinity receptors for several steroid hormones and further that ...Continue Reading

References

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Citations

Jul 9, 1999·Journal of Periodontology·R A GornsteinP Zamorano
Aug 13, 2010·Journal of Periodontal Research·D NebelB-O Nilsson
Dec 18, 2012·Periodontology 2000·Angelo Mariotti, Michael Mawhinney
Dec 2, 2010·Journal of Periodontal Research·D JönssonB-O Nilsson
Apr 16, 2015·Mediators of Inflammation·Min WuShao-Yun Jiang
Jan 1, 1994·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·A Mariotti
Sep 1, 2021·Periodontology 2000·Karen Raju, Lisa Berens

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