Estrogen receptors and human bone cells: immunocytochemical studies.

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
K W ColstonT R Arnett

Abstract

In this immunocytochemical study we have probed a number of human bone cell types and bone preparations for the presence of the estrogen receptor (ER) with two distinct monoclonal antibodies. Using a well-validated antibody (H222) that recognizes human ER and standard peroxidase-antiperoxidase methodology, we were unable to demonstrate nuclear staining for ER in cultured primary or transformed human bone-derived cells or in fetal bone sections. Attempts to visualize ER in osteosarcoma cell lines (TE85C and HTB96) using a silver enhancement procedure were also unsuccessful. Additionally, we failed to detect immunocytochemical staining for the progesterone receptor (using monoclonal antibody mPR1) in control or estrogen-treated human bone cell cultures. Estrogen and progesterone receptor staining was readily detectable in MCF7 human breast cancer cells. In contrast, with a monoclonal antibody that recognizes a 29 kDa cytoplasmic component (p29) closely related to human ER, we observed specific staining in all the osteoblastlike cells studied. Cytoplasmic staining for this p29 antigen was most intense in primary cultures of human bone-derived cells. It is possible that the relatively abundant but as yet undefined p29 antigen may a...Continue Reading

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Citations

Jan 1, 1989·Journal of Steroid Biochemistry·C K WattsR J King
Oct 1, 1993·The Journal of Steroid Biochemistry and Molecular Biology·D K DunnR J King
Jan 1, 1994·Micron : the International Research and Review Journal for Microscopy·G BoivinG Morel
Oct 15, 2013·Medicina oral, patología oral y cirugía bucal·Hugo R Domínguez-MalagónAdalberto Mosqueda-Taylor
Sep 1, 1993·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·A IkegamiH Orimo
Jan 1, 1995·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·I P BraidmanA J Freemont
Aug 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·C K Watts, R J King
Feb 23, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·R Delaveyne-Bitbol, M Garabedian
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Jan 1, 1991·Calcified Tissue International·T C Hillard, J C Stevenson
Sep 1, 1991·Calcified Tissue International·E S OrwollE J Keenan

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