Estrogen regulates somatostatin receptor subtype 2 messenger ribonucleic acid expression in human breast cancer cells

Endocrinology
Y XuJ F Bruno

Abstract

Somatostatin (SRIF) and its analogs exhibit antiproliferative effects that are mediated by SRIF receptors (sst) present in responsive normal and neoplastic tissue including breast cancer. However, information regarding regulation of sst gene expression in cancer cells and modulation of SRIF binding is limited. In the present study we have determined the pattern of sst subtype messenger RNA (mRNA) expression in human breast cancer cells. Furthermore, we investigated the effect of 17beta-Estradiol (E2) treatment on steady state levels of sst mRNA by solution hybridization/nuclease protection analysis and on SRIF binding to membranes of treated cells by receptor binding assay. sst2 mRNA was highly expressed in T47D, ZR75-1, and MDA MB231 cells. Transcripts for sst1 were also detected at very low levels in ZR75-1 cells, whereas sst5 mRNA was expressed at low levels in MCF-7 cells. No sst subtype was detected in MDA MB 435s cells. When the estrogen receptor (ER)(+) cell lines T47D and ZR75-1 were cultured in phenol red-free media plus CS-FCS, sst2 mRNA levels decreased by 60-80% compared with complete serum controls. Adding E2 restored sst2 mRNA levels to control in both cell lines. Moreover, the effect of E2 on sst2 gene expression...Continue Reading

Citations

Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Hanane LaklaiCorinne Bousquet
Jun 1, 1997·Journal of Endocrinological Investigation·Y C Patel
Mar 19, 2004·European Journal of Nuclear Medicine and Molecular Imaging·B Van Den BosscheC Van de Wiele
Oct 1, 2009·PET Clinics·François Bénard, Ayşe Mavi
Aug 6, 1999·Frontiers in Neuroendocrinology·Y C Patel
Apr 16, 1998·International Journal of Cancer. Journal International Du Cancer·K Sharma, C B Srikant
May 22, 2002·Endocrinology·Whitney W WoodmanseeE Chester Ridgway
Oct 28, 1999·Endocrinology·P M Smith-JonesI Virgolini
Feb 18, 2003·Endocrine Reviews·Leo J Hofland, Steven W J Lamberts
Aug 10, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Johannes D VeldhuisCyril Y Bowers
Feb 8, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Leon S FarhyJohannes D Veldhuis
Apr 21, 2001·Annals of Surgical Oncology·J T DolanF C Brunicardi
Apr 30, 1999·Physiological Reviews·E E MüllerD Cocchi
Sep 16, 2017·The Journal of Clinical Endocrinology and Metabolism·Kristin Astrid ØysteseJens Bollerslev
Nov 27, 2020·Journal of the Endocrine Society·Amalina Haydar Ali TajuddinAhmad Marzuki Omar

❮ Previous
Next ❯

Related Concepts