Estrogen regulation of the apolipoprotein AI gene promoter through transcription cofactor sharing.

The Journal of Biological Chemistry
D C HarnishS K Karathanasis

Abstract

Estrogen replacement therapy increases plasma concentrations of high density lipoprotein and its major protein constituent, apolipoprotein AI (apoAI). Studies with animal model systems, however, suggest opposite effects. In HepG2 cells stably expressing estrogen receptor alpha (ERalpha), 17beta-estradiol (E2) potently inhibited apoAI mRNA steady state levels. ApoAI promoter deletion mapping experiments indicated that ERalpha plus E2 inhibited apoAI activity through the liver-specific enhancer. Although the ERalpha DNA binding domain was essential but not sufficient for apoAI enhancer inhibition, ERalpha binding to the apoAI enhancer could not be detected by electrophoretic mobility shift assays. Western blotting and cotransfection assays showed that ERalpha plus E2 did not influence the abundance or the activity of the hepatocyte-enriched factors HNF-3beta and HNF-4, two transcription factors essential for apoAI enhancer function. Expression of the ERalpha coactivator RIP140 dramatically repressed apoAI enhancer function in cotransfection experiments, suggesting that RIP140 may also function as a coactivator on the apoAI enhancer. Moreover, estrogen regulation of apoAI enhancer activity was dependent upon the balance between ER...Continue Reading

References

Aug 1, 1992·Molecular and Cellular Biology·R L WidomS K Karathanasis
Nov 3, 1989·Cell·M Levine, J L Manley
Jul 25, 1989·Nucleic Acids Research·J A LeesM G Parker
Dec 24, 1987·Cell·V KumarP Chambon
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J L BreslowV I Zannis
Jul 1, 1995·Arteriosclerosis, Thrombosis, and Vascular Biology·J K WilliamsT B Clarkson
May 11, 1995·Nucleic Acids Research·S Malik, S Karathanasis
Jan 1, 1994·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·W H EttingerR B Verdery
Oct 1, 1993·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·A KapteinH M Princen
Mar 11, 1993·Nucleic Acids Research·J ChanN C Wong
Apr 30, 1996·Annals of the New York Academy of Sciences·F AuricchioA Rotondi
Aug 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·C L SmithB W O'Malley
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·B HansteinM Brown
Jul 1, 1996·Current Opinion in Cardiology·J W Rich-Edwards, C H Hennekens
Feb 1, 1997·Molecular Endocrinology·A JoyeuxJ C Nicolas
Apr 1, 1997·Current Opinion in Cell Biology·C K GlassM G Rosenfeld

❮ Previous
Next ❯

Citations

Jan 4, 2001·The Journal of Nutritional Biochemistry·H OdaA Yoshida
Nov 17, 2007·The British Journal of Nutrition·Yee M Yuen, Lai K Leung
Mar 29, 2002·The New England Journal of Medicine·Ronald M Krauss
Aug 4, 1999·Current Opinion in Lipidology·H Funke, G Assmann
Mar 16, 2005·Current Opinion in Lipidology·Chao-Qiang LaiJose M Ordovas
Jun 24, 2009·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Huiqing ChenHyunyoung Jeong
Mar 20, 2010·Drugs·Michael J Haas, Arshag D Mooradian
Mar 8, 2014·American Journal of Cardiovascular Drugs : Drugs, Devices, and Other Interventions·Arshag D Mooradian, Michael J Haas
Aug 27, 2013·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Lynne A KellyLucy A Norris
Nov 17, 2006·Amyloid : the International Journal of Experimental and Clinical Investigation : the Official Journal of the International Society of Amyloidosis·Laura ObiciVittorio Bellotti
May 12, 2009·Molecular and Cellular Endocrinology·Yewade NgSally Radovick
Jul 8, 2008·The Journal of Nutritional Biochemistry·Pilaiwan Siripurkpong, Kesara Na-Bangchang
Jan 5, 2011·Biochimica Et Biophysica Acta·Meritxell RosellMalcolm G Parker
Feb 28, 2004·Diabetes·Arshag D MooradianNorman C W Wong
Dec 18, 2007·Biochimica Et Biophysica Acta·Kent R WehmeierMichael J Haas
Oct 18, 2003·Journal of Lipid Research·Stefania Lamon-Fava, Dale Micherone
Jul 26, 2017·Assay and Drug Development Technologies·Michael J HaasArshag D Mooradian
Dec 11, 1999·Arteriosclerosis, Thrombosis, and Vascular Biology·S Lamon-FavaE J Schaefer
Sep 18, 2007·Arteriosclerosis, Thrombosis, and Vascular Biology·Shucun QinMoti L Kashyap
Oct 27, 2001·Circulation Research·M J EvansD C Harnish
Mar 27, 2001·Current Opinion in Lipidology·V I ZannisD Kardassis
May 21, 1999·The Journal of Biological Chemistry·D BoffelliR M Lawn
Jul 5, 2003·The Journal of Biological Chemistry·KehDih LaiMark J Evans
Nov 1, 2005·QJM : Monthly Journal of the Association of Physicians·R OhashiC Chen
Apr 3, 2020·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Mengjing ZhaoBaojian Wu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adrenocortical Carcinoma

Hepatocellular carcinoma is the most common type of primary liver cancer and frequently occurs in individuals with chronic liver diseases like cirrhosis. Here is the latest research.

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.