Estrogen restricts the apoptosis of endometrial stromal cells by promoting TSLP secretion

Molecular Medicine Reports
Hui-Li YangMing-Qing Li

Abstract

Endometriosis (EMS) is a female hormone‑ dependent disease with controversial reports of its etiology and pathogenesis. Apoptosis is particularly important in the human endometrium due to the dynamic cycles of proliferation and shedding. Estrogen possessed antiapoptotic effects on endometrial stromal cells (ESCs), which appears to be exacerbated in women with EMS; however, the underlying mechanism of the antiapoptotic effects of estrogen on ESC remains unknown. The present study aimed to determine whether estrogen regulates the apoptosis of ESCs via thymic stromal lymphopoietin (TSLP) and the associated mechanism. An ELISA was conducted to detect TSLP content in the ESC culture medium treated with estrogen. Subsequently, the early apoptotic rate and expression of B‑cell lymphoma (Bcl‑2) of ESCs were analyzed by flow cytometry in the presence of recombinant human TSLP, anti‑human TSLP neutralizing antibody or estrogen. In the present study, it was reported that ESCs exhibited basal TSLP secretion in the absence of estrogen as reported in previous studies, and that estrogen promoted TSLP secretion of ESCs in a dose‑dependent manner. The results demonstrated that estrogen suppressed the apoptosis of ESCs associated with the promot...Continue Reading

References

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Citations

Mar 4, 2019·Cellular and Molecular Life Sciences : CMLS·Wen-Jie ZhouMing-Qing Li
May 29, 2021·Journal of Molecular Histology·Xinyan WangYan Yu

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Methods Mentioned

BETA
biopsies
ELISA
flow cytometry

Software Mentioned

CellQuest
Becton Dickinson CellQuest
SPSS

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