Estrogen stimulation of 3-o-methyl-D-glucose uptake in isolated rat hepatocytes

Endocrinology
Z MadarF Naftolin

Abstract

The effect of in vivo and in vitro estrogen treatment on 3-O-methyl-D-glucose (30MDG) uptake in isolated rat hepatocytes was examined. A 1-h preincubation of isolated hepatocytes with 17 beta-estradiol (E2) or 17 alpha-ethynyl estradiol stimulated uptake of 30MDG in a dose-dependent fashion. The response appeared to be sterospecific, in that 17 alpha-estradiol was approximately 100-fold less effective than its 17 beta isomer. By itself, the triarylethylene antiestrogen, nafoxidine, slightly increased hepatocyte 30MDG uptake, while in combination with estrogen, nafoxidine completely blocked the effects of both E2 and 17 alpha-ethynyl estradiol. The protein synthesis inhibitor, cycloheximide, inhibited basal 30MDG uptake and abolished the stimulatory effect of estrogen. Kinetic analysis indicated that the estrogen effect resulted from an increase in the Vmax of the 30MDG transport system, with no measurable change in its Km. In vivo estrogen treatment, using either estrogen injections or continuous release Silastic E2 capsules, produced an increase in hepatocyte 30MDG uptake of 52-86%. A similar (66%) increase was seen in pregnant animals between the 14th and 19th days of gestation. These findings demonstrate that estrogens exert...Continue Reading

Citations

Apr 28, 2009·Journal of Physiology and Biochemistry·C I RivasJ C Vera
Jan 1, 1995·Brain Research Bulletin·J Bishop, J W Simpkins
Nov 14, 2003·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Marika MannerströmAnnika Salama
Apr 1, 1992·Reviews in the Neurosciences·J Bishop, J W Simpkins
Feb 19, 2013·Biochimica Et Biophysica Acta·T PiraniA Vieira

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