PMID: 9449243Aug 1, 1997Paper

Estrogen stimulation of creatine kinase B specific activity in 3T3L1 adipocytes after their differentiation in culture: dependence on estrogen receptor

The Journal of Steroid Biochemistry and Molecular Biology
D SömjenA M Kaye

Abstract

We have demonstrated previously that rat adipose tissue showed sex and depot-specific responses to gonadal steroids. The epididymal fat pad in males responded exclusively to androgens by increased specific activity of the brain type isozyme of creatine kinase (CK). In females, the parametrial adipose tissue responded exclusively to estrogens. The present study was undertaken to follow the responsiveness to steroid hormones, and the presence of estrogen receptors (ER), in 3T3L1 cells during their differentiation from pre-adipocytes to adipocytes. In pre-adipocytes in which the basal CK specific activity is low, there was no CK response to 17beta estradiol (E2) or dihydrotestosterone (DHT). Differentiation of the cells into adipocytes was accompanied by increased basal CK activity which was stimulated by E2, but not by DHT. Responsiveness to E2 began 5 days after switching pre-adipocytes to differentiation medium. Upon differentiation, ER became demonstrable in the cell nuclei by staining with FITC labeled anti-idiotypic antibody (clone 1D5) directed against the steroid binding domain of ER. The response to E2 was time-dependent and blocked completely by cycloheximide or actinomycin D. 1D5 itself, which has an estrogen mimetic ef...Continue Reading

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May 1, 1997·The Journal of Steroid Biochemistry and Molecular Biology·D SömjenA M Kaye
Oct 19, 2012·Current Opinion in Clinical Nutrition and Metabolic Care·Labros S Sidossis

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Citations

Dec 1, 1996·The Journal of Steroid Biochemistry and Molecular Biology·D SömjenA M Kaye
Sep 24, 2015·Growth Factors·Biana ShtaifGalia Gat-Yablonski
Jul 14, 2000·Physiological Reviews·M Wyss, R Kaddurah-Daouk

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