Estrogen treatment up-regulates female genes but does not suppress all early testicular markers during rainbow trout male-to-female gonadal transdifferentiation

Journal of Molecular Endocrinology
Denise Vizziano-CantonnetY Guiguen

Abstract

In non-mammalian vertebrates, estrogens are key players in ovarian differentiation, but the mechanisms by which they act remain poorly understood. The present study on rainbow trout was designed to investigate whether estrogens trigger the female pathway by activating a group of early female genes (i.e. cyp19a1, foxl2a, foxl2b, fst, bmp4, and fshb) and by repressing early testicular markers (i.e. dmrt1, nr0b1, sox9a1 and sox9a2). Feminization was induced in genetically all-male populations using 17alpha-ethynylestradiol (EE2, 20 mg/kg of food during 2 months). The expression profiles of 100 candidate genes were obtained by real-time RT-PCR and 45 expression profiles displayed a significant differential expression between control populations (males and females) and EE2-treated populations. These expression profiles were grouped in five temporally correlated expression clusters. The estrogen treatment induced most of the early ovarian differentiation genes (foxl2a, foxl2b, fst, bmp4, and fshb) and in particular foxl2a, which was strongly and quickly up-regulated. Simultaneously, Leydig cell genes, involved in androgen synthesis, as well as some Sertoli cell markers (amh, sox9a2) were strongly repressed. However, in contrast to ou...Continue Reading

Citations

Nov 27, 2009·Fish Physiology and Biochemistry·Jon J AmbergMaria S Sepúlveda
Jan 19, 2012·Biologie aujourd'hui·Adrienne BailletCorinne Cotinot
Mar 20, 2013·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Elizabeth K SmithJ Adam Luckenbach
Sep 27, 2012·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Xiaona ZhangShaoguo Ru
Oct 16, 2012·General and Comparative Endocrinology·Atul K Singh
Oct 16, 2015·Molecular Reproduction and Development·Denise Vizziano-CantonnetIrani Quagio-Grassiotto
Oct 25, 2014·Environmental Science & Technology·Stephen ShortAlex T Ford
Jan 19, 2010·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Martín BlascoDenise Vizziano-Cantonnet
Mar 18, 2009·General and Comparative Endocrinology·Yann GuiguenChing-Fong Chang
Sep 20, 2015·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Talita Sarah MazzoniIrani Quagio-Grassiotto
May 24, 2012·Environmental Toxicology and Chemistry·Yanbin Zhao, Jianying Hu
Apr 23, 2013·General and Comparative Endocrinology·Paulina A BahamondeChristopher J Martyniuk
Aug 28, 2016·Molecular and Cellular Endocrinology·Diego CrespoRüdiger W Schulz
Jul 6, 2021·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Hongjuan ShiGuangli Li
Aug 30, 2021·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Mi OuJian Zhao

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