Estrogenic and antiestrogenic effects of enclomiphene and zuclomiphene on gonadotropin secretion by ovine pituitary cells in culture

Endocrinology
E S Huang, W L Miller

Abstract

17 beta-Estradiol (E2) alters gonadotropin secretions in ovine pituitary cell cultures by 1) augmenting the LH response to LHRH and 2) inhibiting the basal secretion of FSH. These responses were used to study the estrogenic and antiestrogenic effects of enclomiphene, zuclomiphene, and their commercial mixture, Clomid. In terms of the LH response to LHRH, Clomid and enclomiphene (10(-6) M) acted as E2 antagonists because they blocked the action of E2 (10(-10) M). By themselves they did not alter the LHRH response. Previous studies using rat pituitary cultures showed opposite results, since both enclomiphene and Clomid acted as estrogens in rat cultures. Apparently, species differences are involved. One conclusion from these data, therefore, is that it would be unwise to predict how Clomid acts in any species without direct experimentation. Zuclomiphene (10(-7)--10(-5) M) acted as a E2 agonist, in terms of the LH response to LHRH, because it sensitized cultures to LHRH. Surprisingly, all of the clomiphenes, including zuclomiphene, acted primarily as E2 antagonists when FSH secretion was observed. At a concentration of 10(-6) M, they blocked the inhibitory effects of E2 (10(-10 M) on FSH secretion, but enclomiphene and Clomid also...Continue Reading

Citations

Nov 15, 1985·American Journal of Obstetrics and Gynecology·G E SchmidtM H Kim
Apr 1, 1986·American Journal of Obstetrics and Gynecology·G E SchmidtC I Friedman
Aug 19, 2007·Reproductive Biomedicine Online·Shokichi Teramoto, Osamu Kato
Jun 28, 2016·Journal of Acupuncture and Meridian Studies·Abhishek ThakurSugato Banerjee
Jan 1, 1986·Toxicology and Applied Pharmacology·E S Huang, F R Nelson
Jun 24, 2016·Expert Opinion on Pharmacotherapy·Katherine M RodriguezLarry I Lipshultz
May 8, 2019·Expert Review of Endocrinology & Metabolism·Joshua A Earl, Edward D Kim

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.