Estrogenic and toxic effects of polychlorinated biphenyls on cultured ovarian germ cells of embryonic chickens

Reproductive Toxicology
Meina Xie, Caiqiao Zhang

Abstract

Polychlorinated biphenyls (PCBs) are man-made ubiquitous pollutants that have detrimental effects on reproduction and endocrine functions in a variety of species. In the present study, estrogenic and toxic effects of PCBs on embryonic chicken ovarian development were evaluated by a germ-somatic cell co-culture system. Ovarian cells were cultured in serum-free medium and challenged with a mixture of PCBs (Aroclor 1254). Cell proliferation was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) reduction and lactate dehydrogenase (LDH) release. Results showed that lower PCBs (0.1-1 microg/ml) manifested mainly estrogenic effect to stimulate germ cell proliferation, while higher PCBs (10 microg/ml) imposed severe toxicity on germ and somatic cells. The toxic effect of PCBs could be attenuated by an antioxidant tocopherol. PCBs induced condensed nuclear chromosome in ovarian cells and caused cell exfoliation and breakdown within initial hours of treatment. After 24 h, the estrogenic effect of PCBs began to exhibit and the survived germ cells manifested proliferation. Inhibition of the estrogenic effect of PCBs by tamoxifen led to increased toxicity on germ cells and somatic cells. These results indicat...Continue Reading

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Citations

Feb 6, 2010·Birth Defects Research. Part B, Developmental and Reproductive Toxicology·Chunqi Wu
Dec 15, 2004·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Meina XieYuling Mi
May 18, 2005·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Caiqin Zhou, Caiqiao Zhang
Oct 10, 2013·Stem Cell Research·Sittipon Intarapat, Claudio D Stern
Jun 8, 2012·The Science of the Total Environment·Cecilie MiljeteigBjørn Munro Jenssen
Oct 1, 2005·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Yuling MiXinyan Tang
Oct 24, 2006·Analytical and Bioanalytical Chemistry·Sandrine BiauBenoit Roig
Sep 26, 2009·Journal of Proteome Research·Jean-Paul LasserreTorsten Bohn

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