Estrogens induce rapid cytoskeleton re-organization in human dermal fibroblasts via the non-classical receptor GPR30

PloS One
Julie CarnesecchiJean-Marc Vanacker

Abstract

The post-menopausal decrease in estrogen circulating levels results in rapid skin deterioration pointing out to a protective effect exerted by these hormones. The identity of the skin cell type responding to estrogens is unclear as are the cellular and molecular processes they elicit. Here, we reported that lack of estrogens induces rapid re-organization of the human dermal fibroblast cytoskeleton resulting in striking cell shape change. This morphological change was accompanied by a spatial re-organization of focal adhesion and a substantial reduction of their number as evidenced by vinculin and actin co-staining. Cell morphology and cytoskeleton organization was fully restored upon 17β-estradiol (E2) addition. Treatment with specific ER antagonists and cycloheximide respectively showed that the E2 acts independently of the classical Estrogen Receptors and that cell shape change is mediated by non-genomic mechanisms. E2 treatment resulted in a rapid and transient activation of ERK1/2 but not Src or PI3K. We show that human fibroblasts express the non-classical E2 receptor GPR30 and that its agonist G-1 phenocopies the effect of E2. Inhibiting GPR30 through treatment with the G-15 antagonist or specific shRNA impaired E2 effect...Continue Reading

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Citations

Mar 10, 2017·Clinics in Dermatology·Qiang JuWenChieh Chen
Mar 3, 2017·The Journal of Steroid Biochemistry and Molecular Biology·Rosamaria Lappano, Marcello Maggiolini
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Jun 3, 2021·Current Issues in Molecular Biology·Matúš ČomaPeter Gál
Jul 18, 2021·Molecular and Cellular Endocrinology·Ana Paula Santin BertoniTania Weber Furlanetto

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Methods Mentioned

BETA
FCS
transfections
PCR
transfection
hormone replacement therapies

Software Mentioned

ImageJ
PRISM Graphpad
Metamorph

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