Estrous cycle in ob/ob and ovariectomized female mice and its relation with estrogen and leptin

Physiology & Behavior
Ka Yan NgTandra R Chakraborty

Abstract

Hormones like leptin and estrogen have been suggested to increase energy expenditure and modulate estrous cycle. The aim of this study was to investigate the stages of estrous cycle in mouse models with contrasting leptin and estrogen levels. Estrous cycle of wild type (WT), WT ovariectomized (OVX), ob/ob and ob/ob OVX was observed by obtaining vaginal smear and staining with Papanicolaou (PAP) procedure. Quantitative analysis showed cellular morphology and predominance of cellular content across the estrous cycle. Microscopical and quantitative counts showed a 4-5 day regular cycle in WT mouse, WTOVX were acyclic, infertile and hardly showed any cells, ob/ob and ob/ob OVX were both acyclic, infertile and were at persistent estrous or persistent metaestrous phase. Enzyme-Immunometric Assay results showed that leptin levels were about 3 fold higher in WTOVX than WT mouse (p<0.0001) with beta-estradiol values being negligible in WTOVX. The beta-estradiol levels for ob/ob and WT were similar. These results suggest that although leptin and estrogen play an important role in the estrous cycle, they are not the sole factor influencing the cycle. It is probable that in the absence of leptin and decrease in sex steroid hormones with in...Continue Reading

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Citations

Apr 12, 2014·Biochemical Pharmacology·Krisstonia SpruiellMaxwell A Gyamfi
Apr 22, 2017·The Journal of Endocrinology·Russell T TurnerUrszula T Iwaniec
Mar 14, 2018·Journal of the American College of Nutrition·Tandra R ChakrabortySanjoy Chakraborty
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Dec 17, 2020·Journal of the Endocrine Society·Mohammad Lalmoddin MollahYong-Pil Cheon
Apr 27, 2021·Current Protocols·Anna K RadkeSean C Monroe

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