Abstract
The active form of vitamin D, 1 alpha,25-dihydroxyvitamin D3 (VD3), inhibits proliferation and induces differentiation of leukemia cells, but its clinical use is limited by the adverse effect of hypercalcemia. In this study we found that the loop diuretic ethacrynic acid, which is used to treat hypercalcemia, enhanced the differentiation of human leukemia cells induced by VD3. Ethacrynic acid alone inhibited the proliferation of human promyelocytic HL-60 cells while only slightly increasing differentiation markers such as nitroblue tetrazolium (NBT)-reducing and lysozyme activities. Ethacrynic acid effectively enhanced the growth-inhibiting action of VD3. In the presence of ethacrynic acid, VD3 increased the NBT-reducing and lysozyme activities and the CD11b expression of HL-60 cells more effectively than VD3 alone. Other loop diuretics, furosemide and bumetanide, also enhanced the differentiation of HL-60 cells induced by VD3, but to a lesser extent than ethacrynic acid. The differentiation of HL-60 cells induced by all-trans retinoic acid, dimethyl sulfoxide or phorbol-12-myristate 13-acetate was also enhanced by ethacrynic acid with increasing NBT-reducing and lysozyme activities and the expression of CD11b or CD14 surface a...Continue Reading
References
May 15, 1976·International Journal of Cancer. Journal International Du Cancer·C Sundström, K Nilsson
Nov 24, 1977·Nature·S J CollinsR E Gallagher
Feb 1, 1992·Lancet·H AkiyamaY Onozawa
Nov 1, 1992·Endocrine Reviews·D D Bikle
Jan 1, 1991·Leukemia Research·J Okabe-KadoM Hozumi
Dec 21, 1987·FEBS Letters·J AbeY Nishii
Jan 1, 1988·Leukemia Research·T MatsuiT Fujita
Jul 18, 1986·Biochimica Et Biophysica Acta·A HondaY Mori
Feb 12, 1981·Biochemical and Biophysical Research Communications·R W BonserP Cuatrecasas
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·Y HonmaT Suda
Feb 1, 1983·European Journal of Cancer & Clinical Oncology·Y HonmaH Nomura
Jan 1, 1983·Archives of Biochemistry and Biophysics·Y ShiinaT Suda
Sep 1, 1980·Journal of Cellular Physiology·Y HonmaY Koshihara
Aug 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·E AbeT Suda
Oct 15, 1981·Biochemical and Biophysical Research Communications·C MiyauraT Suda
Jan 1, 1981·Annual Review of Pharmacology and Toxicology·D A Koechel
Jan 1, 1995·Leukemia Research·S PakkalaH P Koeffler
May 1, 1993·Leukemia Research·M SakakibaraA Komiyama
Jul 1, 1995·Leukemia & Lymphoma·M Cornic, C Chomienne
Citations
Mar 28, 2009·Journal of Oral Science·Yusuke AmanoMakoto Makishima
Oct 3, 2009·Biomaterials·Jason P MansellAshley W Blom
Feb 5, 2008·Leukemia Research·Akiyoshi HorieYoshio Honma
Feb 10, 1999·Biochemical Pharmacology·M MakishimaY Honma
Sep 27, 2005·Biochemical Pharmacology·Hiroyuki NakanoMakoto Makishima
Jan 24, 2013·PloS One·Shu-Ting ChanShu-Lan Yeh
Mar 18, 2006·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Tetsuro KiyozumiMakoto Kikuchi
Nov 20, 2013·Molecular Medicine Reports·Hai-Shan ZhangYan Wang
Jul 22, 2004·Experimental Cell Research·Michael Danilenko, George P Studzinski