PMID: 8947589Sep 1, 1996Paper

Ethacrynic acid and 1 alpha,25-dihydroxyvitamin D3 cooperatively inhibit proliferation and induce differentiation of human myeloid leukemia cells

Leukemia Research
Makoto Makishima, Y Honma

Abstract

The active form of vitamin D, 1 alpha,25-dihydroxyvitamin D3 (VD3), inhibits proliferation and induces differentiation of leukemia cells, but its clinical use is limited by the adverse effect of hypercalcemia. In this study we found that the loop diuretic ethacrynic acid, which is used to treat hypercalcemia, enhanced the differentiation of human leukemia cells induced by VD3. Ethacrynic acid alone inhibited the proliferation of human promyelocytic HL-60 cells while only slightly increasing differentiation markers such as nitroblue tetrazolium (NBT)-reducing and lysozyme activities. Ethacrynic acid effectively enhanced the growth-inhibiting action of VD3. In the presence of ethacrynic acid, VD3 increased the NBT-reducing and lysozyme activities and the CD11b expression of HL-60 cells more effectively than VD3 alone. Other loop diuretics, furosemide and bumetanide, also enhanced the differentiation of HL-60 cells induced by VD3, but to a lesser extent than ethacrynic acid. The differentiation of HL-60 cells induced by all-trans retinoic acid, dimethyl sulfoxide or phorbol-12-myristate 13-acetate was also enhanced by ethacrynic acid with increasing NBT-reducing and lysozyme activities and the expression of CD11b or CD14 surface a...Continue Reading

References

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Citations

Mar 28, 2009·Journal of Oral Science·Yusuke AmanoMakoto Makishima
Sep 27, 2005·Biochemical Pharmacology·Hiroyuki NakanoMakoto Makishima
Mar 18, 2006·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Tetsuro KiyozumiMakoto Kikuchi
Nov 20, 2013·Molecular Medicine Reports·Hai-Shan ZhangYan Wang
Jul 22, 2004·Experimental Cell Research·Michael Danilenko, George P Studzinski

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