PMID: 3215822Nov 1, 1988Paper

Ethanol accelerates acrosomal loss in human spermatozoa

Journal of Andrology
J G AlvarezB T Storey

Abstract

The effects of ethanol on the loss of the human sperm acrosome, as determined by the chlortetracycline fluorescence assay and by indirect immunofluorescence assay, were assessed over 6 hours during incubation at 37 C in BWW medium containing 0 to 250 mM ethanol. Both assays gave the same results. At the end of 6 hours, 48 +/- 6% acrosomal loss was found in samples in 250 mM ethanol compared with 4 +/- 1% in the absence of ethanol. After 0.25 hour, the first time point chosen for sampling, the spermatozoa in 250 mM ethanol showed 23 +/- 3% loss of acrosomes compared with less than 1% in the absence of ethanol. Ultrastructural studies revealed that the ethanol-treated spermatozoa showed complete acrosomal loss as well as loss of the equatorial segment. No examples of the vesiculation characteristic of the physiologic acrosome reaction were found in the 150 cells examined. Calcium is required for the ethanol-mediated acrosomal loss: omission of Ca2+, addition of 2 mM EGTA, or 0.2 mM verapamil blocked the effect. Ethanol induced a dose-dependent efflux of cholesterol from human spermatozoa, but the ethanol-induced acceleration of acrosomal loss occurred to the same extent in the presence of cholesterol microdispersions that prevent...Continue Reading

References

Jun 1, 1986·Biology of Reproduction·W Byrd, D P Wolf
Feb 1, 1987·Gamete Research·B J RogersW K Vaughn
Jun 1, 1985·Biology of Reproduction·D P WolfK B Bechtol
Feb 1, 1985·Biology of Reproduction·K J Go, D P Wolf
Aug 1, 1974·The New England Journal of Medicine·D H Van Thiel, R Lester
Nov 1, 1974·Experimental Cell Research·R Yanagimachi, N Usui
Jun 1, 1974·Steroids·F M Badr, A Bartke
Sep 1, 1968·The American Journal of Anatomy·J M Bedford
Sep 1, 1967·The Journal of Cell Biology·C BarrosC R Austin
Feb 1, 1981·The Journal of Experimental Zoology·P Talbot, R S Chacon
Feb 1, 1984·Biological Reviews of the Cambridge Philosophical Society·S Meizel
Nov 1, 1984·Developmental Biology·H M FlormanP M Wassarman
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·B K DavisK Bedigian
Jan 1, 1983·Pharmacology, Biochemistry, and Behavior·R A AndersonL J Zaneveld
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·B K Davis
Nov 1, 1982·Biology of Reproduction·R A AndersonL J Zaneveld

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Citations

Apr 27, 2010·Asian Journal of Andrology·Hui WangJia-Hao Sha
Nov 1, 1995·Molecular Reproduction and Development·R C ChianM A Sirard
Apr 18, 2003·Biology of Reproduction·Nicholas L Cross
May 15, 2021·Journal of Animal Science and Technology·Sabina BhandariGaurishankar Manandhar

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