Ethanol Effect on BK Channels is Modulated by Magnesium

Alcoholism, Clinical and Experimental Research
Héctor G MarreroJ R Lemos

Abstract

Alcoholics have been reported to have reduced levels of magnesium in both their extracellular and intracellular compartments. Calcium-dependent potassium channels (BK) are known to be one of ethanol (EtOH)'s better known molecular targets. Using outside-out patches from hippocampal neuronal cultures, we examined the consequences of altered intracellular Mg(2+) on the effects that EtOH has on BK channels. We find that the effect of EtOH is bimodally influenced by the Mg(2+) concentration on the cytoplasmic side. More specifically, when internal Mg(2+) concentrations are ≤200 μM, EtOH decreases BK activity, whereas it increases activity when Mg(2+) is at 1 mM. Similar results are obtained when using patches from HEK cells expressing only the α-subunit of BK. When patches are made with the actin destabilizer cytochalasin D present on the cytoplasmic side, the potentiation caused by EtOH becomes independent of the Mg(2+) concentration. Furthermore, in the presence of the actin stabilizer phalloidin, EtOH causes inhibition even at Mg(2+) concentrations of 1 mM. Internal Mg(2+) can modulate the EtOH effects on BK channels only when there is an intact, internal actin interaction with the channel, as is found at synapses. We propose th...Continue Reading

References

Sep 3, 1998·Alcoholism, Clinical and Experimental Research·M J EckardtB Tabakoff
Nov 7, 2001·The Journal of General Physiology·J Shi, J Cui
May 31, 2002·Nature·Youxing JiangRoderick MacKinnon
Aug 20, 2003·Proceedings of the National Academy of Sciences of the United States of America·Lei HuJianmin Cui
Dec 30, 2003·Proceedings of the National Academy of Sciences of the United States of America·R A Harris, S J Mihic
Sep 17, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Dervla O'MalleyJenni Harvey
Oct 27, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Lijun TianMichael J Shipston
Nov 16, 2006·Biological Research·Ramón Latorre, Sebastian Brauchi
Jan 24, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Gyongyi SzaboStephen B Pruett
Mar 30, 2007·Alcoholism, Clinical and Experimental Research·Paula L Feinberg-Zadek, Steven N Treistman
Jan 2, 2008·The Journal of General Physiology·Frank T Horrigan, Zhongming Ma
Jan 26, 2008·Substance Abuse Treatment, Prevention, and Policy·Kari Poikolainen, Hannu Alho
Jun 10, 2008·Alcoholism, Clinical and Experimental Research·Paula L Feinberg-ZadekSteven N Treistman
Jun 17, 2008·Molecular Pharmacology·Jianxi LiuAlejandro M Dopico
Oct 22, 2008·Nature Structural & Molecular Biology·Huanghe YangJianmin Cui
Nov 5, 2008·Proceedings of the National Academy of Sciences of the United States of America·Gilles E MartinSteven N Treistman
Dec 23, 2008·Cellular and Molecular Life Sciences : CMLS·J CuiU S Lee
Apr 3, 2009·Journal of Molecular Biology·Agnieszka Galińska-RakoczyHanna Strzelecka-Gołaszewska
Apr 25, 2009·Alcoholism, Clinical and Experimental Research·Patrick J MulhollandL Judson Chandler
Jun 27, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Lisa M TorresAndrea Romani
Jul 29, 2010·Trends in Neurosciences·Urvi S Lee, Jianmin Cui
Sep 11, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Ana M RomeroJaime Renau-Piqueras
Sep 15, 2010·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Samanta Oliveira LoureiroRegina Pessoa-Pureur
Oct 15, 2011·Proceedings of the National Academy of Sciences of the United States of America·Victor P T PauBrad S Rothberg
Nov 30, 2011·The Journal of General Physiology·Ren-Shiang ChenKarl L Magleby
Oct 25, 2012·Molecular Pharmacology·Jianxi LiuAlex M Dopico
Oct 24, 2013·Journal of Neurochemistry·Phillip R Gordon-Weeks, Alyson E Fournier
Sep 25, 2014·Frontiers in Neuroanatomy·Michael FrotscherShanting Zhao
Oct 11, 2014·Advances in Neurobiology·Masahiro MoriYoshimi Takai

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Sep 4, 2015·Alcoholism, Clinical and Experimental Research·Frederic Woodward Hopf
Nov 2, 2016·The Journal of General Physiology·Guruprasad Kuntamallappanavar, Alex M Dopico
Jan 31, 2021·International Journal of Molecular Sciences·Michael T McCoyJean Lud Cadet

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