PMID: 9438525Jan 23, 1998Paper

Ethanol effects on cultured embryonic hippocampal neuronal calcium homeostasis are altered by nerve growth factor

Alcoholism, Clinical and Experimental Research
B WebbD W Walker

Abstract

The neurotoxic effect of acute ethanol treatment (AET) may lead to an alteration in the regulation of calcium (Ca2+) homeostasis in hippocampal neurons. Ca2+ homeostasis could be affected by AET when neurons are at rest or after depolarizing activity during synaptic transmission. It has been shown that nerve growth factor (NGF) can ameloriate some types of neurotoxicity by stabilizing Ca2+ homeostasis. Previously, we observed that ethanol (EtOH) changed unstimulated (basal) and potassium (K+)-stimulated intracellular calcium ([Ca2+]i) in embryonic septohippocampal neurons (Webb et al., Brain Res. 729:176-189, 1996). The purpose of the present study is to determine the effects of NGF and EtOH on neuronal Ca2+ homeostasis in cultured embryonic hippocampal neurons. The hypotheses tested were the following: EtOH alters Ca2+ homeostasis in hippocampal neurons; NGF modulates Ca2+ homeostasis in hippocampal neurons; and NGF treatment alters the effect of EtOH on [Ca2+]i in hippocampal neurons. Our results indicated that hippocampal neuronal cultures treated with EtOH had lower basal [Ca2+]i than untreated neurons. EtOH decreased K+-stimulated (30 mM KCI) changes in [Ca2+]i in a dose-dependent manner. During K+ stimulation, 20 ng/ml of...Continue Reading

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Citations

May 24, 2003·Brain Research. Developmental Brain Research·Barbara WebbMarieta B Heaton
Oct 8, 1999·Alcoholism, Clinical and Experimental Research·M J DruseJ L Eriksen
Feb 7, 2012·Experimental Neurology·Pablo BaliñoCarlos M G Aragon
Oct 21, 2016·Alcoholism, Clinical and Experimental Research·Adam J WoodsRonald A Cohen
Nov 1, 2016·Alcohol and Alcoholism : International Journal of the Medical Council on Alcoholism·Liping LiuJian Hu
Jul 21, 2004·Journal of Neuroscience Research·Adrian T McCollum, Steven Estus
Oct 15, 2013·Alcoholism, Clinical and Experimental Research·Pablo BaliñoCarlos M G Aragon

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