PMID: 11923590Mar 30, 2002Paper

Ethanol enhances activation-induced caspase-3 dependent cell death in T lymphocytes

Alcoholism, Clinical and Experimental Research
Sujata KelkarShirish S Barve

Abstract

Clinical and experimental studies have shown that an important deleterious consequence of excessive alcohol consumption is immunosuppression, specifically, a depletion in the mature CD4+ T-cell population. A predominant mechanism involved in T-cell depletion is activation-induced cell death (AICD). Although it is well documented that ethanol intake can cause depletion of CD4+ T cells, the mechanism of how alcohol mediates its effects is unclear. The results were based on data from three separate experiments presented as mean +/- standard deviation (SD). Jurkat CD4+ T cells and peripheral blood lymphocytes were treated with 25 mM of ethanol (12-18 hr), followed by stimulation with mitogens Conconavalin A (5 microg/ml) and Phytohemmaglutinin (1 microg/ml) or T-cell receptor ligation (anti-CD3 antibody (5 microg/ml)) for 6 hr, and then harvested for measurement. The apoptotic cell death markers measured include cell viability, Caspase-3-like activity, and DNA fragmentation. We demonstrate that alcohol pretreatment enhances AICD of Jurkat CD4+ T cells and peripheral blood lymphocytes upon activation by CD3-crosslinking or stimulation with Conconavalin A and Phytohemmaglutinin. Furthermore, we find that the ethanol-mediated enhancem...Continue Reading

References

Feb 15, 1979·Experientia·G P YoungF J Dudley
Jul 10, 1992·Science·L MeyaardF Miedema
Jan 1, 1992·Free Radical Research Communications·R SchreckP A Baeuerle
Aug 1, 1990·Alcoholism, Clinical and Experimental Research·T R JerrellsM J Eckardt
Nov 1, 1989·Proceedings of the Society for Experimental Biology and Medicine·A S Walia, E W Lamon
Aug 1, 1989·Alcoholism, Clinical and Experimental Research·S J Ewald
Feb 1, 1988·Alcoholism, Clinical and Experimental Research·M G Mutchnick, H H Lee
Jan 1, 1987·The Journal of Experimental Medicine·J D AshwellD Hernandez
Sep 1, 1983·The Journal of Experimental Medicine·S C MeuerE L Reinherz
Jan 1, 1995·The Journal of Experimental Medicine·M R AldersonD H Lynch
Feb 2, 1995·Nature·J DheinP H Krammer
Nov 1, 1994·European Journal of Pediatrics·J RajantieM Lanning
Jun 1, 1994·Current Opinion in Immunology·D R Green, D W Scott
Jun 1, 1994·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·N Sheron
Jul 1, 1993·Immunology Today·D KabelitzK Pechhold
May 1, 1993·Seminars in Liver Disease·F Paronetto
Apr 1, 1993·Alcoholism, Clinical and Experimental Research·S J Ewald, H Shao
Apr 1, 1993·Alcoholism, Clinical and Experimental Research·R Domiati-Saad, T R Jerrells
Feb 1, 1996·Clinical and Experimental Immunology·R T CookK McLatchie
Dec 1, 1996·Alcoholism, Clinical and Experimental Research·F J LasoA Orfao
Aug 15, 1997·The Biochemical Journal·G M Cohen
Dec 9, 1997·Cell·G S Salvesen, V M Dixit
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·J D PetersonC Waltenbaugh
Oct 15, 1998·Experimental Cell Research·R PosmanturR B Gilbertsen
Jun 25, 1999·Cell Death and Differentiation·D JiangM J Lenardo
Sep 2, 1999·Alcoholism, Clinical and Experimental Research·F J LasoA Orfao
Nov 18, 2000·The Journal of Laboratory and Clinical Medicine·Q DongS S Barve

❮ Previous
Next ❯

Citations

Sep 25, 2009·Life Sciences·K NurmiK K Eklund
Jul 22, 2009·Liver International : Official Journal of the International Association for the Study of the Liver·Benoit BlanchetAnne Hulin
Nov 24, 2007·Alcoholism, Clinical and Experimental Research·Joaquim Fernandez-SolàAlvaro Urbano-Márquez
Jan 25, 2008·Alcoholism, Clinical and Experimental Research·Khaled AlhomsiMichael Laposata
Sep 9, 2015·Frontiers in Microbiology·Nirupama ChandelPravin C Singhal
Aug 2, 2007·Journal of Clinical Gastroenterology·Eva M RomoEstela Paz-Artal

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis