PMID: 3756540Jul 1, 1986Paper

Ethanol enhances GABA-induced 36Cl-influx in primary spinal cord cultured neurons

Brain Research Bulletin
M K TickuP Lehoullier

Abstract

Ethanol has a pharmacological profile similar to other centrally acting drugs, which facilitate GABAergic transmission. GABA is known to produce its effects by increasing the conductance to Cl- ions. In this study, we have examined the effect of ethanol on GABA-induced 36Cl-influx in primary spinal cord cultured neurons. GABA produces a concentration-dependent, and saturable effect on 36Cl-influx in these neurons. Ethanol potentiates the effect of GABA on 36Cl-influx in these neurons. GABA (20 microM) increased the 36Cl-influx by 75% over the basal value, and in the presence of 50 mM ethanol, the observed increase was 142%. Eadie-Hoffstee analysis of the saturation curves indicated that ethanol decreases the Km value of GABA (10.6 microM to 4.2 microM), and also increases the Vmax. Besides potentiating the effect of GABA, ethanol also appears to have a direct effect in the absence of added GABA. These results suggest that ethanol enhances GABA-induced 36Cl-influx and indicate a role of GABAergic system in the actions of ethanol. These results also support the behavioral and electrophysiological studies, which have implicated GABA systems in the actions of ethanol. The potential mechanism(s) and the role of direct effect of etha...Continue Reading

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Citations

May 4, 2012·Psychopharmacology·Heidi KemppainenKalervo Kiianmaa
Mar 17, 1987·European Journal of Pharmacology·P F Lehoullier, M K Ticku
Jul 17, 1990·European Journal of Pharmacology·A K Mehta, M K Ticku
Jan 3, 1991·European Journal of Pharmacology·G D Frye
Sep 22, 1994·European Journal of Pharmacology·K WegeliusE R Korpi
Sep 5, 1995·European Journal of Pharmacology·P Hyytiä, G F Koob
Aug 1, 1987·Pharmacology, Biochemistry, and Behavior·A M Allan, R A Harris
Oct 1, 1988·Pharmacology, Biochemistry, and Behavior·R J MarleyJ M Wehner
Jun 1, 1988·Pharmacology, Biochemistry, and Behavior·M K Ticku, S K Kulkarni
Feb 1, 1995·Pharmacology, Biochemistry, and Behavior·K L WilliamsG J DiGregorio
Jan 1, 1996·Neuroscience and Biobehavioral Reviews·S S AlmeidaJ R Galler
Jan 1, 1988·Pharmacology & Therapeutics·L A Pohorecky, J Brick
Jan 1, 1990·Pharmacology & Therapeutics·D J Nutt
May 1, 1994·Brain Research. Molecular Brain Research·M Mhatre, M K Ticku
Jan 1, 1991·Progress in Neuro-psychopharmacology & Biological Psychiatry·P L PratherM W Martin
Mar 30, 1992·Neuroscience Letters·M K Sun, D J Reis
Jul 1, 1989·Brain Research Bulletin·B J Bowers, J M Wehner
Jan 1, 1996·Brain Research Bulletin·M DavidsonP A Wilce
Apr 15, 1994·European Journal of Pharmacology·L L Devaud, A L Morrow
Feb 13, 2001·Pharmacology, Biochemistry, and Behavior·O Y VekovischevaE R Korpi
Apr 21, 1999·Brain Research. Brain Research Reviews·A K Mehta, M K Ticku
Aug 15, 2000·Behavioural Brain Research·B SöderpalmJ A Engel

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