Ethanol exposure stimulates cartilage differentiation by embryonic limb mesenchyme cells

Experimental Cell Research
William M Kulyk, L M Hoffman

Abstract

Studies of neural, hepatic, and other cells have demonstrated that in vitro ethanol exposure can influence a variety of membrane-associated signaling mechanisms. These include processes such as receptor-kinase phosphorylation, adenylate cyclase and protein kinase C activation, and prostaglandin production that have been implicated as critical regulators of chondrocyte differentiation during embryonic limb development. The potential for ethanol to affect signaling mechanisms controlling chondrogenesis in the developing limb, together with its known ability to promote congenital skeletal deformities in vivo, prompted us to examine whether chronic alcohol exposure could influence cartilage differentiation in cultures of prechondrogenic mesenchyme cells isolated from limb buds of stage 23-25 chick embryos. We have made the novel and surprising finding that ethanol is a potent stimulant of in vitro chondrogenesis at both pre- and posttranslational levels. In high-density cultures of embryonic limb mesenchyme cells, which spontaneously undergo extensive cartilage differentiation, the presence of ethanol in the culture medium promoted increased Alcian-blue-positive cartilage matrix production, a quantitative rise in 35SO4 incorporatio...Continue Reading

Citations

Apr 22, 2009·Archives of Orthopaedic and Trauma Surgery·Onur HapaGüler Buğdayci
Jul 26, 2011·The British Journal of Nutrition·Malihezaman MonsefiTahereh Talaei-Khozani
Mar 3, 2009·Journal of the Formosan Medical Association = Taiwan Yi Zhi·Hongsen Chiang, Ching-Chuan Jiang
Jul 18, 2008·Medical Hypotheses·Ning ZhangSheng-Jie Xu
Dec 8, 2004·Stem Cells·Boon Chin HengEng Hin Lee
Jul 9, 2010·Alcoholism, Clinical and Experimental Research·Jennifer A HippShay Soker
Aug 23, 2005·Toxicology in Vitro : an International Journal Published in Association with BIBRA·S AdlerS Bremer
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Aug 18, 2004·In Vitro Cellular & Developmental Biology. Animal·Corey S JohnsonE Sidney Hunter

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