Ethanol-induced increase in NADH-dependent monooxygenase activities of hepatic microsomal cytochrome P-450

Alcohol
G Gadeholt, J Mørland

Abstract

NADH-dependent hepatic microsomal monooxygenase activities were measured in the presence and absence of NADPH in material from adult male rats given ethanol in a liquid diet. Controls were given a liquid control diet (control group; lipid as substitute for ethanol) or rat chow (untreated group). Ethanol feeding elevated microsomal aniline hydroxylase activity and did not change ethylmorphine N-demethylase activity. NADH supported 21-24% of the NADPH-driven aniline hydroxylase activity in ethanol, control and untreated microsomes, but only about 6% of ethylmorphine N-demethylase activity. In the presence of NADPH, NADH gave 13-14% increase in aniline hydroxylase activity in microsomes from control and untreated rats, but only 3% in ethanol microsomes. In contrast, the presence of NADPH increased many times the effect of NADH on ethylmorphine N-demethylation with no striking difference between the groups. In another series of experiments, demethylation of 4-nitroanisole was elevated after ethanol feeding (4-fold with NADPH; 5-fold with NADH) and phenobarbital treatment (8-fold with NADPH, 2-fold with NADH). In the ethanol-induced activity, NADH and NADPH were less than additive. In the control and untreated and the phenobarbital-...Continue Reading

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