Ethanol-induced stress response of Staphylococcus aureus

Canadian Journal of Microbiology
Jasmine M PandoJohn E Gustafson

Abstract

Transcriptional profiles of 2 unrelated clinical methicillin-resistant Staphylococcus aureus (MRSA) isolates were analyzed following 10% (v/v) ethanol challenge (15 min), which arrested growth but did not reduce viability. Ethanol-induced stress (EIS) resulted in differential gene expression of 1091 genes, 600 common to both strains, of which 291 were upregulated. With the exception of the downregulation of genes involved with osmotic stress functions, EIS resulted in the upregulation of genes that contribute to stress response networks, notably those altered by oxidative stress, protein quality control in general, and heat shock in particular. In addition, genes involved with transcription, translation, and nucleotide biosynthesis were downregulated. relP, which encodes a small alarmone synthetase (RelP), was highly upregulated in both MRSA strains following ethanol challenge, and relP inactivation experiments indicated that this gene contributed to EIS growth arrest. A number of persistence-associated genes were also upregulated during EIS, including those that encode toxin-antitoxin systems. Overall, transcriptional profiling indicated that the MRSA investigated responded to EIS by entering a state of dormancy and by alterin...Continue Reading

References

Jun 1, 1979·The Journal of Hygiene·H A LillyA Zaggy
Jan 1, 1984·Journal of Bacteriology·K M Dombek, L O Ingram
Sep 19, 1998·FEMS Microbiology Letters·M W QoronflehB J Wilkinson
Jan 9, 1999·Clinical Microbiology Reviews·G McDonnell, A D Russell
Apr 3, 2001·Applied and Environmental Microbiology·C Barker, S F Park
Dec 26, 2001·Nucleic Acids Research·Ron EdgarAlex E Lash
Jan 22, 2004·FEMS Immunology and Medical Microbiology·Ambrose L CheungYan-Qiong Xiong
Apr 28, 2004·FEMS Microbiology Reviews·Stéphane BronnerGilles Prévost
Jun 16, 2005·Journal of Clinical Microbiology·Frances G O'BrienJohn E Gustafson
Aug 12, 2005·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Fred M GordinJanet A Gill
Oct 21, 2005·Journal of Bacteriology·Maria M SennBrigitte Berger-Bächi
Feb 3, 2006·Journal of Bacteriology·Wook ChangWilliam E Bentley
Apr 7, 2006·Applied and Environmental Microbiology·Indranil ChatterjeeMathias Herrmann
Oct 13, 2006·Archives of Internal Medicine·Susan S HuangRichard Platt
Oct 24, 2006·Journal of Bacteriology·James T RiordanJohn E Gustafson
Oct 4, 2007·BMC Genomics·Vijayaraj Nagarajan, Mohamed O Elasri
Nov 11, 2009·Microbial Pathogenesis·Moshe KoremMel Rosenberg
Dec 30, 2009·Journal of Bacteriology·Niles P DoneganAmbrose L Cheung
May 29, 2010·Microbiology and Molecular Biology Reviews : MMBR·Zachary D DalebrouxMichele S Swanson
Jun 2, 2010·Clinics in Laboratory Medicine·Yi-Wei Tang, Charles W Stratton
Jan 7, 2011·Molecular Microbiology·Alexander K W ElsholzUlf Gerth
Nov 23, 2011·Antimicrobial Agents and Chemotherapy·Swantje ReissSusanne Engelmann
Mar 7, 2012·Antimicrobial Agents and Chemotherapy·Ying ZhangMichael R Barer
Mar 14, 2012·Antimicrobial Agents and Chemotherapy·Yang SongBrian J Wilkinson
Aug 25, 2012·Frontiers in Cellular and Infection Microbiology·Rosmarie GauppGreg A Somerville
Feb 28, 2013·BMC Genomics·Jose Antonio IbarraLindsey N Shaw
Jun 19, 2013·Cell Host & Microbe·Nadia R CohenJames J Collins

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Nihon rinsho. Japanese journal of clinical medicine
Teruyo Ito, Keiichi Hiramatsu
© 2022 Meta ULC. All rights reserved