PMID: 11923596Mar 30, 2002Paper

Ethanol-induced translocation of protein kinase A occurs in two phases: control by different molecular mechanisms

Alcoholism, Clinical and Experimental Research
D P DohrmanIvan Diamond

Abstract

Cyclic adenosine monophosphate (cAMP)-dependent protein kinase A (PKA) regulates cellular functions. The specificity of PKA-mediated phosphorylation is determined primarily by PKA localization to sub-cellular sites. Chronic exposure to ethanol causes sustained translocation of the PKA catalytic subunit (C) from the Golgi to the nucleus in NG108-15 cells. Here we find that this is preceded by a transient short-term ethanol-induced translocation of PKA C. Different molecular mechanisms appear to underlie early and late phases of ethanol-induced translocation of PKA subunits. The time course and localization of PKA C and regulatory (RII) subunits was assessed by immunocytochemistry in NG108-15 cells in the presence of ethanol, adenosine receptor (A2) blockade, and inhibitors of PKA activity and RNA and protein synthesis. Ethanol induces an early phase (<30 min) of C translocation to the cytoplasm and nucleus. This requires cAMP via adenosine A2 receptor activation. C then returns to the Golgi area after 60 min. A second phase of C translocation occurs during continuing exposure to ethanol (>12 hr). Re-accumulation of nuclear C no longer requires A2 or cAMP. RII also translocates to the nucleus during chronic treatment with ethanol...Continue Reading

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Citations

Jan 20, 2004·Pharmacology & Therapeutics·William S Mailliard, Ivan Diamond
Mar 20, 2004·Pharmacology & Therapeutics·Sandeep KumarA Leslie Morrow
Jan 29, 2005·Proceedings of the National Academy of Sciences of the United States of America·Tieqiao WenPing Shen
Feb 18, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Cristina Velázquez-MarreroSteven N Treistman
Sep 15, 2004·Alcoholism, Clinical and Experimental Research·Maria Pia ArolfoPatricia H Janak
Dec 15, 2004·Alcoholism, Clinical and Experimental Research·Jon R FeeTodd E Thiele
Nov 11, 2008·Annals of the New York Academy of Sciences·Anna M Lee, Robert O Messing
Apr 26, 2006·Alcoholism, Clinical and Experimental Research·Frank M FerraroTodd E Thiele
Sep 11, 2007·European Journal of Pain : EJP·Irina VetterPeter J Cabot
Feb 15, 2011·Alcoholism, Clinical and Experimental Research·Kenneth S KendlerPablo V Gejman
Oct 22, 2016·Frontiers in Psychiatry·Nicole Mons, Daniel Beracochea

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