Ethanol induces cytostasis of cortical basal progenitors

Journal of Biomedical Science
Amanjot Kaur RiarLenin Mahimainathan

Abstract

Developing brain is a major target for alcohol's actions and neurological/functional abnormalities include microencephaly, reduced frontal cortex, mental retardation and attention-deficits. Previous studies have shown that ethanol altered the lateral ventricular neuroepithelial cell proliferation. However, the effect of ethanol on subventricular basal progenitors which generate majority of the cortical layers is not known. We utilized spontaneously immortalized rat brain neuroblasts obtained from cultures of 18-day-old fetal rat cerebral cortices using in vitro ethanol exposures and an in utero binge model. In the in vitro acute model, cells were exposed to 86 mM ethanol for 8, 12 and 24 h. The second in vitro model comprised of chronic intermittent ethanol (CIE) exposure which consisted of 14 h of ethanol treatment followed by 10 h of withdrawal with three repetitions. E18 neuroblasts expressing Tbr2 representing immature basal progenitors displayed significant reduction of proliferation in response to ethanol in both the models. The decreased proliferation was accompanied by absence of apoptosis or autophagy as illustrated by FACS analysis and expression of apoptotic and autophagic markers. The BrdU incorporation assay indica...Continue Reading

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Citations

Dec 6, 2017·International Journal of Molecular Sciences·Dhyanesh PatelGeorge Henderson
May 23, 2018·International Journal of Molecular Sciences·Dhyanesh PatelMadhusudhanan Narasimhan
Dec 2, 2020·Acta Neuropathologica Communications·Florent MarguetAnnie Laquerrière

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Methods Mentioned

BETA
FACS
protein assay
electrophoresis
flow cytometry
transgenic

Software Mentioned

CELL
Vi
Scion Image
Adobe Photoshop
GraphPad Instat
Cell analyzer
FACSDiva
FlowJo

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