PMID: 9449229Feb 4, 1998Paper

Ethanol induces subtype-specific up-regulation of muscarinic acetylcholine receptor mRNA in neurohybrid cell lines

Life Sciences
F FukamauchiD M Chuang

Abstract

Effects of sub-chronic ethanol treatment on the mRNA levels of muscarinc acetylcholine receptor (mAChR) subtypes were studied in two neurohybrid cell lines, NG108-15 and NCB-20. The former expresses only m4-mAChRs, while the latter expresses m1- and m4-mAChRs. Exposure to 200 mM ethanol for 1 to 3 days induced a time-dependent increase in the levels of m4-mAChR mRNA in NG108-15 and NCB-20 cells. In contrast, the levels of ml-mAChR mRNA and mAChR-mediated phosphoinositide hydrolysis in NCB-20 cells were unchanged. The ethanol-induced up-regulation of m4-mAChR mRNA was associated with a parallel increase in the levels of mAChR binding sites assessed by using [3H]quinuclidinyl benzilate. The mRNA up-regulation was closely correlated with a decrease in intracellular cyclic AMP concentration and the ethanol up-regulation was blocked by 8-bromo-cyclic AMP and forskolin, suggesting that m4-mAChR mRNA is under negative regulation by cyclic AMP. Thus, neurally-related cell lines are useful models for studying molecular mechanisms underlying ethanol-induced alteration of mAChR expression and function in the nervous system.

References

Aug 1, 1990·Biochemical Pharmacology·C S RabeB Tabakoff
Dec 29, 1995·The Journal of Biological Chemistry·I C WoodN J Buckley
Mar 23, 2011·Antimicrobial Agents and Chemotherapy·Robert H BarkerEdmund Sybertz

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