PMID: 9540844Apr 16, 1998Paper

Ethanol induces the expression of alpha 1(I) procollagen mRNA in a co-culture system containing a liver stellate cell-line and freshly isolated hepatocytes

Biochimica Et Biophysica Acta
L FontanaM Rojkind

Abstract

To study the fibrogenic action of ethanol in vitro we used a co-culture system of freshly isolated hepatocytes and a liver stellate cell line (CFSC-2G) developed in our laboratory. Our results show that in this co-culture system ethanol induces the expression of alpha 1(I) procollagen mRNA in a dose- and time-dependent manner. This effect of ethanol was due to its metabolism by alcohol dehydrogenase since 4-methylpyrazole prevented the ethanol-mediated increase in alpha 1(I) procollagen mRNA. Ethanol was more efficient than acetaldehyde in inducing alpha 1(I) procollagen mRNA expression and its effect was protein synthesis-independent. Transfection of either hepatocytes or liver stellate cells with a reporter gene, chloramphenicol acetyl transferase (CAT), driven by 3700 bp of the mouse alpha 1(I) procollagen promoter demonstrated that only LSC expressed significant CAT activity and that this activity was enhanced by ethanol. Overall, our results suggest that this co-culture system is a useful model to study alcohol-induced fibrogenesis in vitro and that mechanisms other than acetaldehyde formation may also play an important role in alcohol-induced fibrogenesis.

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Citations

Jun 7, 2011·Pharmaceutical Research·Ravi S ShuklaKun Cheng
Aug 31, 2002·Journal of Gastroenterology and Hepatology·Minoti Apte
Jun 17, 1999·Alcoholism, Clinical and Experimental Research·P Greenwel
Aug 19, 2011·BMC Gastroenterology·Ancha BaranovaZobair M Younossi
Dec 8, 2010·Toxicology and Applied Pharmacology·Subhadip GhatakAmal Santra
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Nov 17, 2006·World Journal of Gastroenterology : WJG·Jian-Hua WangJacob George
Oct 15, 2020·Biomolecules·Mohamed ElnagdyLeila Gobejishvili
Feb 10, 2007·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M Van de BovenkampP Olinga
May 22, 2003·The American Journal of Pathology·Benjamin SchaeferMarcos Rojkind

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