Ethanol inhibition of reticulocyte protein synthesis: the role of haem

British Journal of Haematology
M L FreedmanF J Forte

Abstract

Ethanol, in concentrations of 0.05-0.8 M, inhibited intact human and rabbit reticulocyte protein synthesis in the presence of iron-transferrin for endogenous haem synthesis. Associated with this effect there was a conversion of polyribosomes to monoribosomes and a decreased incorporation of radioactive leucine into nascent globin chains. When physiological levels of ethanol (0.05-0.1 M) were used, these effects were prevented by incubation with 50 muM haemin and reversed by removing the alcohol and reincubating with iron-transferrin or haemin. The polyribosomal disaggregation was also prevented by stopping ribosomal movement with 5 mM cycloheximide. Neither ATP nor GSH levels were altered in the presence of ethanol. When non-physiological levels of 0.8 M ethanol were used, haemin did not prevent the inhibition of protein synthesis. Likewise, in the rabbit reticulocyte cell-free lysate system containing haemin inhibition was noted at concentrations greater than 0.05 M ethanol. The polyribosomal disaggregation in reticulocytes incubated with 0.8 M ethanol was associated with decreased dissociation of monoribosomes into subunits. Similarly, when ribosomes were directly suspended cell-free in 0.1 or 0.8 M ethanol there was a decrea...Continue Reading

References

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Citations

Oct 1, 1987·Applied Biochemistry and Biotechnology·M A Findeis, G M Whitesides
Jul 29, 1988·Clinica Chimica Acta; International Journal of Clinical Chemistry·L R Solomon
Mar 1, 1976·The Journal of Clinical Investigation·M L Freedman, J Rosman
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May 1, 1977·Postgraduate Medicine·J B Green, A A Trobridge
Jan 1, 1981·Alcoholism, Clinical and Experimental Research·J W Ostroff

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