PMID: 2117442Jul 15, 1990Paper

Ethanol inhibits thrombin-induced secretion by human platelets at a site distinct from phospholipase C or protein kinase C

The Biochemical Journal
C Benistant, R Rubin

Abstract

Ethanol is known to inhibit the activation of platelets in response to several physiological agonists, but the mechanism of this action is unclear. The addition of physiologically relevant concentrations of ethanol (25-150 mM) to suspensions of washed human platelets resulted in the inhibition of thrombin-induced secretion of 5-hydroxy[14C]tryptamine. Indomethacin was included in the incubation buffer to prevent feedback amplification by arachidonic acid metabolites. Ethanol had no effect on the activation of phospholipase C by thrombin, as determined by the formation of inositol phosphates and the mobilization of intracellular Ca2+. Moreover, ethanol did not interfere with the thrombin-induced formation of diacylglycerol or phosphatidic acid. Stimulation of platelets with phorbol ester (5-50 nM) resulted in 5-hydroxy[14C]tryptamine release comparable with those with threshold doses of thrombin. However, ethanol did not inhibit phorbol-ester-induced secretion. Ethanol also did not interfere with thrombin- or phorbol-ester-induced phosphorylation of myosin light chain (20 kDa) or a 47 kDa protein, a known substrate for protein kinase C. By electron microscopy, ethanol had no effect on thrombin-induced shape change and pseudopod ...Continue Reading

Citations

Oct 1, 1995·Alcoholism, Clinical and Experimental Research·M L RandR L Kinlough-Rathbone
Dec 15, 2004·Alcoholism, Clinical and Experimental Research·Dylan W de LangeAlbert van de Wiel
Apr 1, 1992·Lipids·C D Stubbs, R Rubin
Feb 1, 1994·Alcoholism, Clinical and Experimental Research·L D SnellP L Hoffman
Feb 1, 1994·Alcoholism, Clinical and Experimental Research·R Rubin, M L Rand
Jun 15, 2007·Alcoholism, Clinical and Experimental Research·Dylan W de LangeJan-Willem N Akkerman
Aug 17, 2005·American Journal of Clinical Pathology·Raneem O Salem, Michael Laposata
Dec 21, 2004·European Journal of Clinical Investigation·D W de LangeA van de Wiel
Dec 1, 1996·Alcoholism, Clinical and Experimental Research·R A DeitrichK Gill
Jun 15, 1995·Clinica Chimica Acta; International Journal of Clinical Chemistry·D M GoldbergJ G Parkes
May 24, 1996·Chemistry and Physics of Lipids·S Cockcroft
Aug 10, 2010·Alcohol·Mikio Marumo, Ichiro Wakabayashi

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