PMID: 2494946Apr 1, 1989Paper

Ethanol interferes with collagen-induced platelet activation by inhibition of arachidonic acid mobilization

Archives of Biochemistry and Biophysics
R Rubin

Abstract

The effect of ethanol on signal generation in collagen-stimulated human platelets was evaluated. Incubation of washed human platelets with physiologically relevant concentrations of ethanol (25-150 mM) resulted in a dose-dependent inhibition of aggregation and secretion in response to collagen (0.5-10 micrograms/ml), but did not inhibit shape change. In platelets labeled with [3H]arachidonic acid, ethanol significantly inhibited the release of arachidonic acid from phospholipids, in both the presence and the absence of indomethacin. Thromboxane B2 formation was also inhibited in proportion to the reduction in free arachidonic acid. There was a close correlation between the extent of inhibition of arachidonic acid release and secretion. The inhibition of platelet aggregation and secretion by ethanol was partially overcome by the addition of exogenous arachidonic acid. In the presence of indomethacin, ethanol had no effect on the activation of phospholipase C by collagen as determined by the formation of inositol phosphates and phosphatidic acid. Moreover, ethanol had no effect on the mobilization of intracellular calcium by collagen and only minimally inhibited the early phases of the phosphorylation of myosin light chain (20 kD...Continue Reading

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Citations

Nov 1, 1991·Lipids·J L HojnackiJ A Jakubowski
Apr 1, 1992·Lipids·C D Stubbs, R Rubin
Dec 18, 2008·Archives of Toxicology·Glaecir R Mundstock DiasVera Maria Morsch
Jan 3, 1994·Biochimica Et Biophysica Acta·J BalsindeJ A Solís-Herruzo
Mar 15, 1996·Clinica Chimica Acta; International Journal of Clinical Chemistry·S C Renaud, J C Ruf
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Feb 1, 1994·Alcoholism, Clinical and Experimental Research·R Rubin, M L Rand
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Jul 9, 1999·Alcoholism, Clinical and Experimental Research·R Rubin
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