Ethanol production via in situ fungal saccharification and fermentation of mild alkali and steam pretreated corn fiber

Bioresource Technology
Prachand ShresthaJ Hans van Leeuwen

Abstract

The effect of mild alkali and steam pretreatments on fungal saccharification and sequential simultaneous-saccharification and fermentation (SSF) of corn fiber to ethanol was studied. The corn fiber was pretreated with: (i) 2% NaOH (w/w) at 30 degrees C for 2h and (ii) steaming at 100 degrees C for 2h. Ethanol yields were 2.6g, 2.9g and 5.5g ethanol/100g of corn fiber, respectively, for Phanerochaete chrysosporium, Gloeophyllum trabeum and Trichoderma reesei saccharification and sequential SSFs. SSF with commercial cellulase enzyme - Spezyme-CP had 7.7g ethanol/100g corn fiber. Mild alkali pretreatment resulted in higher glucose yields following fungal saccharification of corn fiber. However, the ethanol yields were comparatively similar for untreated and mild alkali pretreated corn fiber. Solid-substrate fermentation of corn fiber with fungi can be improved to either eliminate or reduce the dosage of commercial cellulase enzymes during SSF.

References

Apr 1, 2004·Applied Biochemistry and Biotechnology·Beatriz Palmarola-AdradosGuido Zacchi
Aug 12, 2004·Applied Biochemistry and Biotechnology·B C Saha, R J Bothast
Dec 14, 2004·Bioresource Technology·Nathan MosierMichael Ladisch
Feb 28, 2006·Trends in Genetics : TIG·Jure PiskurConcetta Compagno
Apr 19, 2008·Applied Biochemistry and Biotechnology·Bernard A Prior, Donal F Day
May 15, 2008·Journal of Agricultural and Food Chemistry·Prachand ShresthaJ Hans van Leeuwen
Jul 11, 2009·Biotechnology and Bioengineering·Jin Seop BakKyoung Heon Kim
May 27, 2009·Journal of Agricultural and Food Chemistry·Prachand ShresthaJ Hans van Leeuwen

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Citations

Dec 26, 2012·Bioresource Technology·Yeshitila A TsigieYi-Hsu Ju
Jun 21, 2011·Applied and Environmental Microbiology·Prachand ShresthaJohn W Taylor
May 8, 2014·Biotechnology Progress·Yanna LiangSabrina Trupia
Jul 2, 2011·Bioresource Technology·Richard L GilesMatthew W Parrow

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