PMID: 3245893Nov 1, 1988Paper

Ethanol promotes hydrolysis of 3H-labeled sialoconjugates from brain of mice in vitro

Alcohol
J Mathew, W R Klemm

Abstract

Acute administration of ethanol reportedly decreases total sialic acid in brain. Here, we tested the hypothesis in brain and liver that the decrement is due to increased hydrolysis of sialoglycoconjugates. Mouse tissue slices were pulse-labeled with N-[3H]acetyl-D-mannosamine, the precursor of sialic acid. Incorporation was linear for up to 4 hr of incubation. When the labeled slices were incubated with three concentrations of ethanol (0.1, 0.5, and 1 M) for 5 hr, labeled liver sialoconjugates were significantly affected only at 0.5 and 1 M ethanol, whereas labeled brain sialoconjugates were markedly decreased even at 100 mM ethanol. Sialidase activity decreased steadily with increasing concentration of ethanol, indicating that the increased hydrolysis was not attributable to an enhanced sialidase activity. n-Propanol and t-butanol had the same degradative effect as ethanol on sialocompounds; and 3 mM pyrazole, an inhibitor of alcohol dehydrogenase (ADH), had no effect on ethanol-induced degradation of sialocompounds. The protein/DNA ratio in liver showed a steady decrease with increasing ethanol. The data thus confirm the in vivo reports of ethanol-enhanced cleavage and rule out any increase in sialidase activity as a major ca...Continue Reading

References

Jan 1, 1979·Journal of Neuroscience Research·W R Klemm, R L Engen
Jan 1, 1978·Journal of Neuroscience Research·W R Klemm, R L Engen
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T B BeisswengerB L Vallee
Jan 1, 1988·Life Sciences·W R KlemmL Cherian
Jan 1, 1987·Progress in Neuro-psychopharmacology & Biological Psychiatry·W R Klemm
May 1, 1988·Alcohol·W R KlemmR G Maring
Sep 8, 1986·Life Sciences·W R Klemm, D M Foster
Nov 15, 1974·Brain Research·L Tauc, D H Hinzen
Sep 27, 1974·Biochimica Et Biophysica Acta·A PerinA Arnaboldi
Oct 1, 1971·Journal of Neurochemistry·R H Quarles, R O Brady
Aug 15, 1984·Clinica Chimica Acta; International Journal of Clinical Chemistry·O VesterbergD Schmidt
Jan 1, 1982·Alcoholism, Clinical and Experimental Research·R C BakerR A Deitrich
Oct 1, 1981·Journal of Neurochemistry·C A LandaR Caputto
Jul 1, 1980·Brain Research Bulletin·G I HattonC D Tweedle

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Citations

Aug 1, 1989·Pharmacology, Biochemistry, and Behavior·J Mathew, W R Klemm
Jun 1, 1991·Pharmacology, Biochemistry, and Behavior·F SchellenbergJ Weill
Jan 1, 1990·Alcohol·W R Klemm
Oct 7, 2011·Traffic Injury Prevention·Evelyn VingilisEdward M Adlaf
Oct 2, 1998·Alcoholism, Clinical and Experimental Research·P GhoshE A Hale
May 1, 1988·Alcohol·W R KlemmR G Maring

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