Ethanol transiently suppresses choline-acetyltransferase in basal nucleus of Meynert slices.

Brain Research
Daniela EhrlichChristian Humpel

Abstract

The cholinergic system plays a major role in learning and cognition and cholinergic neurons appear to be particularly vulnerable to ethanol (EtOH) exposure. There are conflicting results if EtOH directly damages cholinergic neurons. Thus, the aims of the present study were (1) to investigate the effect of different EtOH concentrations on cholinergic neurons in organotypic brain slices of the nucleus basalis of Meynert (nbM) and (2) to study if the most potent cholinotrophic substance nerve growth factor (NGF) or inhibitors of mitogen activated kinase (MAPK) p38- and nitric-oxide synthase (NOS)-pathways may counteract any EtOH effect. Two-week old organotypic rat brain slices of the nbM were exposed to 1-100 mM EtOH for 7 days with or without drugs and the number of choline-acetyltransferase (ChAT)-positive neurons was counted. Our data show that EtOH significantly reduced the number of ChAT-positive neurons with the most potent effect at a concentration of 50 mM EtOH (54±5 neurons per slice, p<0.001), compared to control slices (120±13 neurons per slice). Inhibition of MAPK p38 (SB 203580, 10 μM) and NOS (L-thiocitrulline, 10 μM) counteracted the EtOH-induced decline of cholinergic neurons and NGF protected cholinergic neurons ...Continue Reading

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Methods Mentioned

BETA
ELISAs
dissection
ELISA

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