PMID: 9428690Jan 15, 1998Paper

Ethanolamine, but not phosphoethanolamine, potentiates the effects of insulin, phosphocholine, and ATP on DNA synthesis in NIH 3T3 cells--role of mitogen-activated protein-kinase-dependent and protein-kinase-independent mechanisms

European Journal of Biochemistry
Z KissT Chung

Abstract

NIH 3T3 fibroblasts express a phospholipase D activity hydrolyzing phosphatidylethanolamine (PtdEtn) which produces ethanolamine (Etn) in response to a variety of growth regulating agents. The main objective of this work was to evaluate the effects of Etn on mitogenesis and to determine whether these effects require its metabolism to phosphoethanolamine (PEtn) or PtdEtn. To increase conversion of Etn to PEtn, an Etn-specific kinase derived from Drosophila was highly expressed in NIH 3T3 cells. Overexpression of this Etn kinase resulted in large (10-12.5-fold) increases in PEtn formation, but only in modest (1.2-1.7-fold) increases in PtdEtn synthesis. In both vector control and Etn kinase overexpressor cells, Etn had biphasic effects on insulin-induced DNA synthesis with maximal (approximately 2-fold) potentiating effects being observed at 0.5-1 mM concentrations, followed by an inhibitory phase at higher Etn concentrations. In the Etn kinase overexpressor lines, the inhibitory phase was elicited by lower Etn concentrations and it was partially blocked by 5 mM choline due to decreased formation of PEtn. In both vector control and Etn kinase overexpressor cells, phosphocholine (PCho) and insulin synergistically stimulated DNA sy...Continue Reading

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Citations

Jun 9, 2005·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Yuki KariyaYasuyuki Igarashi
Mar 16, 2005·Developmental Biology·Alberto PascualThomas Préat
Jan 12, 2002·Journal of Cellular Biochemistry·Itsuki AjiokaYoshifumi Watanabe
Jan 18, 2007·The Journal of Biological Chemistry·Devorah MatasZvi Vogel
Sep 22, 2020·American Journal of Physiology. Endocrinology and Metabolism·Serene JosephMaureen Keller-Wood

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