Ethanolamine modulates DNA synthesis through epidermal growth factor receptor in rat primary hepatocytes.

In Vitro Cellular & Developmental Biology. Animal
Hisae Kume, Hajime Sasaki

Abstract

Ethanolamine (Etn) stimulates hepatocyte proliferation in vivo and in vitro; however, the physiological function of Etn in hepatocytes has yet to be elucidated. In the present study, we examined the effect of Etn using a primary culture of rat hepatocytes. The level of membrane phosphatidylethanolamine (PE) significantly decreased when the hepatocytes were cultured without Etn but increased to the level found in the liver when the culture medium was supplemented with 20- 50 microM Etn. Moreover, Etn stimulated DNA synthesis in a dose-dependent manner and had a synergistic effect with epidermal growth factor (EGF). A binding assay and Western blotting showed that the number of EGF receptors was 22- 30% lower in cells grown in the absence of Etn compared to those grown in its presence, but the respective Kd values were almost the same. Furthermore, tyrosine phosphorylation of the EGF receptor was significantly lower in cells grown without Etn. Phosphatidylcholine (PC) synthesis in the liver is unique in that it occurs via stepwise methylation of PE. We found that without Etn supplementation, bezafibrate-induced inhibition of PE methylation increased the level of PE by decreasing its conversion to PC and stimulated DNA synthesis. ...Continue Reading

References

Dec 1, 1990·Journal of Cellular Physiology·T Kano-SueokaS J Klug
Feb 1, 1986·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·D M Peehl, T A Stamey
Sep 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·S L HammondM R Stampfer
Oct 1, 1983·Journal of Cellular Physiology·T Kano-SueokaL A Walsh
Feb 1, 1982·Journal of Cellular Physiology·M C TsaoR G Ham
Nov 1, 1981·Experimental Cell Research·T Kano-Sueoka, J E Errick
Jul 8, 1997·Proceedings of the National Academy of Sciences of the United States of America·H SasakiN Takahashi
Dec 16, 1997·Proceedings of the National Academy of Sciences of the United States of America·C J WalkeyD E Vance
May 30, 1998·In Vitro Cellular & Developmental Biology. Animal·H SasakiN Takahashi
Jan 5, 2002·In Vitro Cellular & Developmental Biology. Animal·T Kano-SueokaJ K Kawamoto

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Citations

Apr 10, 2007·Trends in Molecular Medicine·Padmavathi Bandhuvula, Julie D Saba
Apr 17, 2016·In Vitro Cellular & Developmental Biology. Animal·Huansheng YangYulong Yin
Mar 30, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Rachid JellaliEric Leclerc

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