Ethanolic leaf extract of Coccinia grandis is effective against both drug resistant and drug sensitive clinical isolates of Indian Kala-azar

Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology
Sangita LahiryMadhumita Manna

Abstract

The emergence of resistance to the current available drugs used for treatment against Indian Kala-azar (KA) or Visceral Leishmaniasis makes the control strategy inadequate for the disease. This grave epidemiological situation directed researches towards alternative treatments including herbal therapy. In this background, the aim of the present study was to evaluate the antileishmanial activity of the leaves of Coccinia grandis (a tropical vine) against both the Sodium Stibo Gluconate (SSG) sensitive and resistant as well as Miltefosine (MIL) sensitive and resistant field isolates of Leishmania donovani. The cytotoxicity effect of ethanolic extract of leaves of C. grandis (Cg-LE) against the clinical isolates of L. donovani was checked both in promastigotes and intracellular amastigotes stages. In both sensitive and resistant promastigotes, Cg-LE stimulated reactive oxygen species generation and apoptosis. Parasites infected macrophages showing enhanced nitric oxide production after Cg-LE treatment suggested the leishmanicidal activity of the leaf extract. Furthermore, Cg-LE treatment led to mitochondrial membrane damage and DNA fragmentation in promastigotes. The present study is very encouraging for the fact that Cg-LE showed ...Continue Reading

References

Oct 1, 1982·Analytical Biochemistry·L C GreenS R Tannenbaum
Aug 3, 1994·Biochemical Pharmacology·A T Evans, S L Croft
Oct 26, 2000·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·S SundarH W Murray
Sep 7, 2001·Fitoterapia·P B de Carvalho, E I Ferreira
Nov 13, 2001·Tropical Medicine & International Health : TM & IH·S Sundar
Jan 5, 2002·Medical Microbiology and Immunology·S Sundar
Feb 1, 2002·Natural Product Reports·M J Chan-Bacab, L M Peña-Rodríguez
Feb 9, 2002·Current Opinion in Microbiology·Amal O Amer, Michele S Swanson
Feb 28, 2002·Current Pharmaceutical Design·Simon L Croft, Vanessa Yardley
Nov 29, 2002·The New England Journal of Medicine·Shyam SundarJonathan Berman
May 27, 2003·Apoptosis : an International Journal on Programmed Cell Death·J D LyA Lawen
Jul 1, 2004·Comparative Immunology, Microbiology and Infectious Diseases·P Desjeux
Jul 27, 2004·Antimicrobial Agents and Chemotherapy·Navin K Verma, Chinmoy S Dey
Jan 19, 2006·Clinical Microbiology Reviews·Simon L CroftAlan H Fairlamb
Feb 20, 2007·Parasitology International·Deivid Costa SoaresElvira Maria Saraiva
Mar 15, 2008·Parasitology Research·A Abdul Rahuman, P Venkatesan
Jul 8, 2010·Journal of Global Infectious Diseases·Lukasz Kedzierski
Sep 30, 2010·Antimicrobial Agents and Chemotherapy·Stephan KrembRuth Brack-Werner
Jul 1, 2008·Disability and Health Journal·Nicole S BellPaul J Amoroso
Oct 17, 2012·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Fernanda Cristina Macedo RondonHilton César Rodrigues Magalhães

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Mar 17, 2020·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Sangita LahiryMadhumita Manna

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