Ethidium cooperativity in the formation of complexes with CpG

Biochemical and Biophysical Research Communications
M Day, C G Trowbridge

Abstract

The formation of complexes between the self-complementary ribo-dinucleoside monophosphate CpG and ethidium ion is observed by use of an ethidium ion selective electrode. The ratio of total CpG to total ethidium was varied from 50:1 to .4:1, with CpG concentrations ranging from 0.2 to 1.1 mM. Scatchard plots show that the system is strongly cooperative with respect to ethidium ion; cooperativity with respect to dinucleoside has been previously reported (Krugh, T.R., Wittlin, F.N., and Cramer, S.P. (1975) Biopolymers 14,197-210). Cooperative behavior with respect to ethidium ion implies the existence of complexes containing at least two molecules of ethidium ion in combination with one or more CpG molecules.

Citations

Oct 7, 2004·Analytical Biochemistry·Francesco MalatestaMarcella Venturini
May 14, 2016·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Ayaka SeichiKoji Suzuki

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