Ethyl Acetate Fraction of Dicliptera chinensis (L.) Juss. Ameliorates Liver Fibrosis by Inducing Autophagy via PI3K/AKT/mTOR/p70S6K Signaling Pathway.

Chinese Journal of Integrative Medicine
Yuan LiuZhi-Ping Lyu

Abstract

To investigate the molecular mechanism underlying the anti-hepatic fibrosis activity of ethyl acetate fraction Dicliptera chinensis (L.) Juss. (EDC) in human hepatic stellate cells (HSCs) in vitro and in a carbon tetrachloride (CCl4)-induced hepatic fibrosis mouse model in vivo. For in vitro study, HSCs were pre-treated with platelet-derived growth factor (10 ng/mL) for 2 h to ensure activation and treated with EDC for 24 h and 48 h, respectively. The effect of EDC on HSCs was assessed using cell counting kit-8 assay, EdU staining, transmission electron microscopy, immunofluorescence staining, and Western blot, respectively. For in vivo experiments, mice were intraperitoneally injected with CCl4 (2 ° L/g, adjusted to a 25% concentration in olive oil), 3 times per week for 6 weeks, to develop a hepatic fibrosis model. Forty 8-week-old male C57BL/6 mice were divided into 4 groups using a random number table (n=10), including control, model, positive control and EDC treatment groups. Mice in the EDC and colchicine groups were intragastrically administered EDC (0.5 g/kg) or colchicine (0.2 mg/kg) once per day for 6 weeks. Mice in the control and model groups received an equal volume of saline. Biochemical assays and histological ex...Continue Reading

References

Jul 29, 2006·Autophagy·Daniel J KlionskyPatrice Codogno
Feb 29, 2008·Nature·Noboru MizushimaDaniel J Klionsky
Jul 2, 2008·Journal of Gastroenterology·Natalie J Török
Jun 30, 2010·Nature Reviews. Gastroenterology & Hepatology·Scott L Friedman
Nov 5, 2011·Cell Death and Differentiation·D DentonS Kumar
Nov 8, 2011·Arab Journal of Gastroenterology : the Official Publication of the Pan-Arab Association of Gastroenterology·Amani N ShafikMohamed E Mahmoud
Sep 7, 2012·Cold Spring Harbor Perspectives in Biology·Brian A Hemmings, David F Restuccia
Sep 15, 2012·Annals of the Rheumatic Diseases·Neng-Yu LinJörg H W Distler
Jan 12, 2013·Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association·Don C Rockey
Mar 15, 2013·Journal of Hepatology·Virginia Hernández-GeaScott L Friedman
Apr 30, 2013·Current Opinion in Microbiology·Keith B Boyle, Felix Randow
Jul 19, 2013·Biochemical Society Transactions·Michael A Destefano, Estela Jacinto
Nov 7, 2013·Nature Reviews. Gastroenterology & Hepatology·Jaime L Schneider, Ana Maria Cuervo
Dec 11, 2013·Cell Research·Peidu Jiang, Noboru Mizushima
Jan 3, 2014·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jian SunJinlin Hou
Jul 22, 2014·Liver International : Official Journal of the International Association for the Study of the Liver·Jie LiCheng Guo
Oct 4, 2014·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Jiang-Wei ChenLei-Sheng Jiang
Feb 15, 2015·Gut·Youngmin A LeeScott L Friedman
Mar 19, 2015·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Patrice N MimcheTracey J Lamb
Apr 22, 2015·Chinese Medicine·Shao-Ru ChenYi-Tao Wang
May 12, 2015·Journal of Pineal Research·Beatriz San-MiguelJavier González-Gallego
Jul 28, 2016·The Journal of Toxicological Sciences·Shu DongShi-Bing Su
Mar 28, 2017·International Journal of Biological Macromolecules·Yourui XuKefeng Zhang

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