Ethyl acetate fraction of Radix rubiae inhibits cell growth and promotes terminal differentiation in cultured human keratinocytes

Journal of Ethnopharmacology
Lin-Li ZhouZhong Zuo

Abstract

In Chinese medicine practice, Radix rubiae, the dry root of Rubia cordifolia L. is commonly used for the treatment of psoriasis. Psoriasis is a chronic inflammatory skin disorder characterized by hyperproliferation and aberrant differentiation of epidermal keratinocytes. Our previous studies identified Radix rubiae to have potent antiproliferative action on cultured HaCaT keratinocytes and to induce keratinocyte differentiation in mouse tail model. The present study aimed to investigate whether Radix rubiae could also induce terminal differentiation in cultured human keratinocytes. The cornified envelope (CE) formation assay showed that ethyl acetate (EA) fraction of Radix rubiae significantly accentuated the CE formation, a well-recognized marker of terminal differentiation, in cultured HEK and HaCaT cells in a dose and time dependent manner. Western blot analyses demonstrated that EA fraction of Radix rubiae at a concentration of 3.2μg/ml significantly increased transglutaminase type I and involucrin expression in both HEK and HaCaT keratinocytes after 96 h treatment, a response similar to that of Ca²⁺ positive control. Moreover, the expression level of cytokeratin 5/14, which is specifically related to cell proliferation, wa...Continue Reading

References

Jan 1, 1988·Virchows Archiv. A, Pathological Anatomy and Histopathology·J KanitakisJ Thivolet
Aug 1, 1983·Canadian Journal of Biochemistry and Cell Biology = Revue Canadienne De Biochimie Et Biologie Cellulaire·F C Stevens
Sep 1, 1981·The Journal of Cell Biology·S Banks-Schlegel, H Green
Oct 1, 1995·The British Journal of Dermatology·I M LeighP N Riddle
Mar 1, 1995·Clinics in Dermatology·I A McKay, I M Leigh
Mar 2, 1995·The New England Journal of Medicine·M W Greaves, G D Weinstein
Apr 1, 1996·Cell Biology International·K S RaoP D Gupta
May 20, 1998·European Journal of Cell Biology·D BreitkreutzN E Fusenig
Oct 20, 1998·Journal of the American Academy of Dermatology·M DuvicS Mays
Nov 26, 1998·Archives of Dermatology·J Koo, S Arain
Jul 21, 1999·Proceedings of the National Academy of Sciences of the United States of America·Z NemesP M Steinert
Aug 15, 2000·Skin Pharmacology and Applied Skin Physiology·B SebökH Gollnick
Sep 3, 2003·Clinical and Experimental Dermatology·T W Tse
Apr 2, 2005·Nature Reviews. Molecular Cell Biology·Eleonora CandiGerry Melino
Sep 24, 2005·Experimental Dermatology·Daniel Hohl
Jan 6, 2007·The Journal of Investigative Dermatology·Eva M SchindlerTatiana Efimova
May 12, 2007·Toxicology and Applied Pharmacology·Sivaprakasam Balasubramanian, Richard L Eckert
Jul 7, 2009·International Journal of Molecular Medicine·Wai-Pui TseZhi-Xiu Lin
Jul 25, 2009·The Journal of Investigative Dermatology·Juan A PenaJennifer L Gooch
Feb 16, 2010·The Journal of Steroid Biochemistry and Molecular Biology·Bodo LehmannMichael Meurer
Jul 27, 2010·Journal of Cutaneous Pathology·Wael I Al-DarajiBernhard Zelger
Nov 16, 2010·Phytotherapy Research : PTR·Do Youn JunYoung Ho Kim

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