Ethyl Pyruvate Increases Post-Ischemic Levels of Mitochondrial Energy Metabolites: A 13 C-Labeled Cerebral Microdialysis Study

Metabolites
Kevin Heebøll NygaardAxel Forsse

Abstract

Mitochondrial dysfunction after transient cerebral ischemia can be monitored by cerebral microdialysis (CMD) using changes in the lactate and pyruvate concentrations and ratio. Other metabolites associated with mitochondrial (dys)function are, e.g., tricyclic acid (TCA) and purine metabolites. Ethyl pyruvate (EP) is a putative neuroprotectant, supposedly targeting mitochondrial energy metabolism, but its effect on cerebral energy metabolism has never been described using microdialysis. In this study we monitored the metabolic effects of EP in the endothelin-1 (ET-1) rat model using perfusion with 13C-succinate and analysis of endogenous and 13C-labeled metabolites in the dialysates by liquid chromatography-mass spectrometry (LC-MS). Adult Sprague Dawley rats (n = 27 of which n = 11 were included in the study) were subjected to the microdialysis experiments. Microdialysis probes were perfused with 13C-labeled succinate (1 mM), and striatal dialysates were collected at 30 min intervals before induction of the insult, during intracerebral application of ET-1, and during intravenous treatment with either EP (40 mg/kg) or placebo, which was administered immediately after the insult. The rats were subjected to transient cerebral isch...Continue Reading

References

Jul 1, 1997·Metabolism: Clinical and Experimental·T YamamotoK Higashino
Jul 9, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sarah HolguinRichard Wurtman
Jan 28, 2009·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Bolin CaiLuis Ulloa
Mar 19, 2009·American Journal of Physiology. Endocrinology and Metabolism·Emma HeartPeter J S Smith
Feb 2, 2010·The International Journal of Biochemistry & Cell Biology·Piero Luigi IpataFrancesco Balestri
Jun 8, 2012·Journal of Neurochemistry·Sara CiprianiMichael A Schwarzschild
Oct 31, 2012·International Journal of Molecular Sciences·Jens MinnerupChristoph Kleinschnitz
May 7, 2014·Acta Neurologica Scandinavica·A JacobsenC H Nordström
May 23, 2014·Journal of Neurology, Neurosurgery, and Psychiatry·Karol P BudohoskiPeter J Kirkpatrick
Oct 16, 2015·Acta Neurochirurgica. Supplement·Devin W McBrideJohn H Zhang
Nov 20, 2015·Neuropharmacology·Felicita PedataAnna Maria Pugliese
Jan 8, 2016·Redox Biology·Celia QuijanoAndrés Trostchansky
Oct 16, 2016·Critical Care : the Official Journal of the Critical Care Forum·Charles L Francoeur, Stephan A Mayer
Nov 1, 2016·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Ibrahim JallohKeri Lh Carpenter
Jul 5, 2017·Frontiers in Neurology·Carl-Henrik NordströmMagnus Olivecrona
Oct 14, 2017·Acta neurochirurgica·Carl-Henrik Nordström

❮ Previous
Next ❯

Citations

Dec 29, 2020·CNS Neuroscience & Therapeutics·Leilei MaoHong Shi

❮ Previous
Next ❯

Software Mentioned

STATA
- view
NDP

Related Concepts

Related Feeds

Basal Ganglia

Basal Ganglia are a group of subcortical nuclei in the brain associated with control of voluntary motor movements, procedural and habit learning, emotion, and cognition. Here is the latest research.